Location of Repository

The Role of Tensin in Cell Migration and Fibronectin Fibrillogenesis

By Jonathan David Howe


The tensin family of cytoskeletal-associated proteins are implicated in fibronectin fibrillogenesis and integrin-mediated cell migration. There are four members of the human tensin family. Tensin1, 2 and 3 are large proteins (~150-200 kDa) with widespread expression in adult tissues that are thought to couple integrins to the actin cytoskeleton, whereas tensin4 (cten) is a smaller protein (77 kDa) with a restricted expression profile that is not thought to interact with actin.\ud To study the role of tensin in fibronectin fibrillogenesis, which is mediated by fibrillar adhesions, I first characterised the isoform specificities of several anti-tensin antibodies. These antibodies were then used to determine the tensin expression profile of human foreskin fibroblasts (HFFs), which were found to express tensin1, 2 and 3. Unfortunately, most of the antibodies did not work sufficiently well for immunofluorescence microscopy so the sub-cellular localisation of the tensins was largely investigated using GFP-tagged proteins. Tensin1, 2 and 3 localised to both focal and fibrillar adhesions, although relative distribution between these two adhesion types was isoform-specific. Since tensin3 preferentially localised to fibrillar adhesions, I used an siRNA approach to investigate its role in fibronectin fibrillogenesis, and found that tensin3 was required for this process.\ud Although tensins play an important role in cell migration, it is unclear whether they act as positive or negative regulators. Therefore, I used over-expression and siRNAmediated knockdown to clarify the role of tensin1, 2 and 3 in both 2D and 3D migration. Interestingly, I found that modulating tensin expression did not affect the 2D migration of HFF, HEK293, or A2780 ovarian cancer cells. However, all three tensins were required for the 3D migration of A2780 cells in fibronectin-containing microenvironments.\ud Over-expressing Rab25, a small GTPase up-regulated in aggressive ovarian cancers, in A2780 cells promotes invasive migration in 3D microenvironments by recycling and retaining integrin at the cell front (Caswell et al., 2007). Tensin was required for the invasive migration and morphology of Rab25-expressing A2780 cells. However, tensin depletion did not affect global α5 integrin recycling or the retention of photoactivated GFP-α5 integrin at the cell front. Interestingly, Rab25 over-expression was also found to promote tensin-dependent fibronectin fibrillogenesis in A2780 cells, suggesting that tensin promotes 3D migration by facilitating fibronectin fibril remodelling

Publisher: University of Leicester
Year: 2010
OAI identifier: oai:lra.le.ac.uk:2381/7951

Suggested articles



  1. (2007). A chemical approach to the identification of tensinbinding proteins.
  2. (1995). A dual functional signal mediator showing RhoGAP and phospholipase C-delta stimulating activities.
  3. (1995). A mechanical function of myosin II in cell motility.
  4. (2007). A molecular switch that controls cell spreading and retraction.
  5. (2000). A novel RGD-independent fibronectin assembly pathway initiated by alpha4beta1 integrin binding to the alternatively spliced V region.
  6. (2003). A novel role for FAK as a protease-targeting adaptor protein: regulation by p42 ERK and Src.
  7. (2007). A paxillin tyrosine phosphorylation switch regulates the assembly and form of cell-matrix adhesions.
  8. (2004). A PLCdelta1-binding protein, p122/RhoGAP, is localized in caveolin-enriched membrane domains and regulates caveolin internalization.
  9. (2004). A PLCdelta1-binding protein, p122RhoGAP, is localized in focal adhesions.
  10. (2009). A primary role for Golgi positioning in directed secretion, cell polarity, and wound healing.
  11. (1986). A re-examination of the interaction of vinculin with actin.
  12. (2007). A reciprocal tensin-3-cten switch mediates EGF-driven mammary cell migration.
  13. (1998). A role for Cdc42 in macrophage chemotaxis.
  14. (2001). A role of tensin in skeletal-muscle regeneration.
  15. (1998). A screen to identify Drosophila genes required for integrin-mediated adhesion.
  16. (2005). A specific alpha5beta1-integrin conformation promotes directional integrin translocation and fibronectin matrix formation.
  17. (2000). A structural basis for integrin activation by the cytoplasmic tail of the alpha IIb-subunit.
  18. (2002). A structural mechanism of integrin alpha(IIb)beta(3) "inside-out" activation as regulated by its cytoplasmic face.
  19. (1954). A syndrome of intra-uterine dwarfism recognizable at birth with cranio-facial dysostosis, disproportionately short arms, and other anomalies (5 examples).
  20. (2008). Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner.
  21. (2007). Actin stress fibres.
  22. Activation of endogenous Cdc42 visualized in living cells.
  23. (2006). Activation of leukocyte beta2 integrins by conversion from bent to extended conformations.
  24. (2002). Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells.
  25. (1975). Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy.
  26. (1999). Agoniststimulated cytoskeletal reorganization and signal transduction at focal adhesions in vascular smooth muscle cells require c-Src.
  27. (2006). Alphavbeta6 integrin in wound healing and cancer of the oral cavity.
  28. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
  29. (1998). Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7.
  30. (2008). Analyzing real-time PCR data by the comparative C(T) method.
  31. (1968). Anchorage and growth regulation in normal and virus-transformed cells.
  32. (2006). and Src dependence distinguishes mesenchymal and amoeboid modes of tumour cell invasion: a link to integrin function.
  33. (2002). Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites.
  34. (2007). Association of the tensin N-terminal protein-tyrosine phosphatase domain with the alpha isoform of protein phosphatase-1 in focal adhesions.
  35. (2008). Asymmetric focal adhesion disassembly in motile cells.
  36. (1997). Autosomal mutations affecting adhesion between wing surfaces in Drosophila melanogaster.
  37. (2009). beta1 integrin cytoplasmic domain residues selectively modulate fibronectin matrix assembly and cell spreading through talin and Akt-1.
  38. (2003). Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins.
  39. (2007). Big roles for small GTPases in the control of directed cell movement.
  40. (1983). Binding of plasma fibronectin to cell layers of human skin fibroblasts.
  41. (2003). blistery encodes Drosophila tensin protein and interacts with integrin and the JNK signaling pathway during wing development.
  42. (1996). Breaking the integrin hinge. A defined structural constraint regulates integrin signaling.
  43. (2009). C-terminal Tensin-like (CTEN) is an oncogene which alters cell motility possibly through repression of E-cadherin in colorectal cancer.
  44. (2005). C1-TEN is a negative regulator of the Akt/PKB signal transduction pathway and inhibits cell survival, proliferation, and migration.
  45. (1999). Calpain cleavage of integrin beta cytoplasmic domains.
  46. (1996). Calreticulin modulates cell adhesiveness via regulation of vinculin expression.
  47. (2008). Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex.
  48. (1995). Capping protein levels influence actin assembly and cell motility in dictyostelium.
  49. (2003). Caspase-dependent cleavage of tensin induces disruption of actin cytoskeleton during apoptosis.
  50. (2005). Caveolin-1 in oncogenic transformation, cancer, and metastasis.
  51. (2003). Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity.
  52. (2004). Cdc42--the centre of polarity.
  53. (2006). Cell and fibronectin dynamics during branching morphogenesis.
  54. (2002). Cell interactions with threedimensional matrices.
  55. (2005). Cell migration at a glance.
  56. (2005). Cell migration during gastrulation.
  57. (2005). Cell migration in 3D matrix.
  58. (1996). Cell migration: a physically integrated molecular process.
  59. (2003). Cell migration: integrating signals from front to back.
  60. (2004). Cell migration: mechanisms of rear detachment and the formation of migration tracks.
  61. (2004). Cell migration: Rho GTPases lead the way.
  62. (2008). Cell motility through plasma membrane blebbing.
  63. (1993). Cell spreading on extracellular matrix proteins induces tyrosine phosphorylation of tensin.
  64. (1997). Cell to substratum adhesion is involved in v-Src-induced cellular protein tyrosine phosphorylation: implication for the adhesion-regulated protein tyrosine phosphatase activity.
  65. (1984). Cell-substratum interaction of cultured avian osteoclasts is mediated by specific adhesion structures.
  66. (2003). Cellular motility driven by assembly and disassembly of actin filaments.
  67. (1991). Characterization of actin filament severing by actophorin from Acanthamoeba castellanii.
  68. (2005). Characterization of segments from the central region of BRCA1: an intrinsically disordered scaffold for multiple protein-protein and protein-DNA interactions?
  69. (1995). Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase.
  70. (2004). Chemoattractant signaling in dictyostelium discoideum.
  71. (2005). Cleavage of cten by caspase-3 during apoptosis.
  72. (2007). Cleavage of tensin during cytoskeleton disruption in YTX-induced apoptosis.
  73. (1998). Cloning, characterization, and chromosomal localization of a gene frequently deleted in human liver cancer (DLC-1) homologous to rat RhoGAP.
  74. (2009). Collective cell migration in morphogenesis, regeneration and cancer.
  75. (1987). Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion.
  76. (2008). Comparative dynamics of retrograde actin flow and focal adhesions: formation of nascent adhesions triggers transition from fast to slow flow.
  77. (2003). Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis.
  78. Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation.
  79. (1995). Consequences of lack of beta 1 integrin gene expression in mice.
  80. (1997). Control of actin dynamics in cell motility.
  81. (1999). Control of actin dynamics in cell motility. Role of ADF/cofilin.
  82. (2001). Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa.
  83. (1999). Cooperation between mDia1 and ROCK in Rho-induced actin reorganization.
  84. (2002). Coupling of folding and binding for unstructured proteins.
  85. (2002). Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand.
  86. (2001). Crystal structure of the extracellular segment of integrin alpha Vbeta3.
  87. (2003). Cten mRNA expression is correlated with tumor progression in thymoma.
  88. (2003). Cten mRNA expression was correlated with tumor progression in lung cancers.
  89. (2002). Cten, a COOH-terminal tensin-like protein with prostate restricted expression, is down-regulated in prostate cancer.
  90. (2010). CTEN/tensin 4 expression induces sensitivity to paclitaxel in prostate cancer.
  91. (2008). Cytogenetic and molecular characterization of a de-novo t(2p;7p) translocation involving TNS3 and EXOC6B genes in a boy with a complex syndromic phenotype.
  92. (2004). Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion.
  93. (1993). Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin.
  94. (2005). Defining and assaying RNAi in mammalian cells.
  95. (1999). Degraded collagen fragments promote rapid disassembly of smooth muscle focal adhesions that correlates with cleavage of pp125(FAK), paxillin, and talin.
  96. (2003). Deleted in liver cancer (DLC) 2 encodes a RhoGAP protein with growth suppressor function and is underexpressed in hepatocellular carcinoma.
  97. (2009). Deleted in liver cancer 1 (DLC1) utilizes a novel binding site for Tensin2 PTB domain interaction and is required for tumor-suppressive function.
  98. (2007). Deleted in liver cancer 3 (DLC-3), a novel Rho GTPase-activating protein, is downregulated in cancer and inhibits tumor cell growth.
  99. (2008). Deleted in liver cancer-1 (DLC-1): a tumor suppressor not just for liver.
  100. (1995). Deletion of beta 1 integrins in mice results in inner cell mass failure and peri-implantation lethality.
  101. (1996). Differential effects of platelet-derived growth factor isotypes on human smooth muscle cell proliferation and migration are mediated by distinct signaling pathways.
  102. (2007). Differential transmission of actin motion within focal adhesions.
  103. (2003). Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis.
  104. (2003). Dimensions and dynamics in integrin function.
  105. (1994). Disruption of epithelial cell-matrix interactions induces apoptosis.
  106. (2003). Disruption of the Diaphanous-related formin Drf1 gene encoding mDia1 reveals a role for Drf3 as an effector for Cdc42.
  107. (2007). Dissecting focal adhesions in cells differentially expressing calreticulin: a microscopy study.
  108. (2003). DLC-1 gene inhibits human breast cancer cell growth and in vivo tumorigenicity.
  109. (2004). DLC-1 operates as a tumor suppressor gene in human nonsmall cell lung carcinomas.
  110. (2005). DLC-1, a Rho GTPase-activating protein with tumor suppressor function, is essential for embryonic development.
  111. (2008). DLC1 is a chromosome 8p tumor suppressor whose loss promotes hepatocellular carcinoma.
  112. (2002). Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants.
  113. (2006). Dynamic study of the transition from hyaluronan- to integrin-mediated adhesion in chondrocytes.
  114. (2000). Dynamics and segregation of cellmatrix adhesions in cultured fibroblasts.
  115. (1992). Dynamics of beta 1 integrin-mediated adhesive contacts in motile fibroblasts.
  116. (2003). Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells.
  117. (2003). Effects of thrombin on interactions between beta3-integrins and extracellular matrix in platelets and vascular cells.
  118. (1988). Electron microscopy and structural model of human fibronectin receptor.
  119. (2004). Epidermal growth factor modulates tyrosine phosphorylation of a novel tensin family member, tensin3.
  120. (2000). Epidermal growth factor receptor activation of calpain is required for fibroblast motility and occurs via an ERK/MAP kinase signaling pathway.
  121. (1985). Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling.
  122. (2009). FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST.
  123. (2004). FAK promotes organization of fibronectin matrix and fibrillar adhesions.
  124. (2004). FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly.
  125. (2002). Fibronectin at a glance.
  126. (2002). Fibronectin extension and unfolding within cell matrix fibrils controlled by cytoskeletal tension.
  127. (2008). Fibronectin fibrillogenesis regulates threedimensional neovessel formation.
  128. (2005). Fibronectin fibrillogenesis, a cell-mediated matrix assembly process.
  129. (2004). Fibronectin matrix assembly regulates alpha5beta1-mediated cell cohesion.
  130. (1990). Fibronectin matrix deposition and fibronectin receptor expression in healing and normal skin.
  131. (2002). Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions.
  132. (2003). Fibronectin requirement in branching morphogenesis.
  133. (1990). Fibronectin-rich fibrillar extracellular matrix controls cell migration during amphibian gastrulation.
  134. (2001). Fibronectin, integrins, and growth control.
  135. (1993). Fibulin's organization into the extracellular matrix of fetal lung fibroblasts is dependent on fibronectin matrix assembly.
  136. (2008). Filopodia: molecular architecture and cellular functions.
  137. (2001). Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontin.
  138. (2009). Fluorescent labeling techniques for investigation of fibronectin fibrillogenesis (labeling fibronectin fibrillogenesis).
  139. (2004). Focal adhesion and actin dynamics: a place where kinases and proteases meet to promote invasion.
  140. (1999). Focal adhesion motility revealed in stationary fibroblasts.
  141. (2006). Focal adhesions: paradigm for a signaling nexus.
  142. (2006). Focal adhesions: what's new inside.
  143. (1985). Focal contacts of normal and RSV-transformed quail cells. Hypothesis of the transformationinduced deficient maturation of focal contacts.
  144. (2008). Follow the monomer.
  145. (1994). Fos-transformation activates genes associated with invasion.
  146. (2006). From the membrane to the nucleus and back again: bifunctional focal adhesion proteins.
  147. (2007). Functional atlas of the integrin adhesome.
  148. (2001). Functional consequences of integrin gene mutations in mice.
  149. (2005). GDIs: central regulatory molecules in Rho GTPase activation.
  150. (2005). GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors.
  151. (2002). Gene structure, tissue expression, and linkage mapping of the mouse DLC-1 gene (Arhgap7).
  152. (2007). Getting to the site of inflammation: the leukocyte adhesion cascade updated.
  153. (2006). Glanzmann thrombasthenia.
  154. (2002). Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling.
  155. (1995). Growth cone advance is inversely proportional to retrograde F-actin flow.
  156. (1998). Growth hormone stimulates the formation of a multiprotein signaling complex involving p130(Cas) and CrkII. Resultant activation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK).
  157. (1998). Growth hormone stimulates the tyrosine phosphorylation and association of p125 focal adhesion kinase (FAK) with JAK2. Fak is not required for stat-mediated transcription.
  158. (2004). Hierarchical assembly of cell-matrix adhesion complexes.
  159. (1989). Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
  160. (1985). Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
  161. (2004). Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors.
  162. (1996). Identification of a new biological function for the integrin alpha v beta 3: initiation of fibronectin matrix assembly.
  163. (2008). Identification of genes that regulate epithelial cell migration using an siRNA screening approach.
  164. (1998). Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration.
  165. (2004). ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells.
  166. (2006). ILK, PINCH and parvin: the tIPP of integrin signalling.
  167. (2005). Immune cell migration in inflammation: present and future therapeutic targets.
  168. (2005). Immunobiology : the immune system in health and disease, 6th edn
  169. (1982). Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts.
  170. (2005). Inactivation of tensin3 in mice results in growth retardation and postnatal lethality.
  171. (1995). Increased tyrosine phosphorylation of focal adhesion proteins in myeloid cell lines expressing p210BCR/ABL.
  172. (1998). Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP.
  173. (1990). Inhibition of binding of fibronectin to matrix assembly sites by anti-integrin (alpha 5 beta 1) antibodies.
  174. (1992). Inhibition of neutrophil chemokinesis on vitronectin by inhibitors of calcineurin.
  175. (2002). Initial stages of cell-matrix adhesion can be mediated and modulated by cell-surface hyaluronan.
  176. (2008). Ins and outs of ADF/cofilin activity and regulation.
  177. (1995). Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix.
  178. (2004). Integrin activation.
  179. (2002). Integrin adhesion: when is a kinase a kinase?
  180. (2003). Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling.
  181. (2000). Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis.
  182. (1996). Integrin dynamics on the tail region of migrating fibroblasts.
  183. (2006). Integrin ligands at a glance.
  184. (2009). Integrin signalling at a glance.
  185. (2000). Integrin structure.
  186. (2006). Integrin trafficking and the control of cell migration.
  187. (1996). integrin-dependent and -independent polymerization of fibronectin.
  188. (1997). Integrin-ligand binding properties govern cell migration speed through cellsubstratum adhesiveness.
  189. (2006). Integrin-linked kinase and its partners: a modular platform regulating cell-matrix adhesion dynamics and cytoskeletal organization.
  190. (2001). Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta.
  191. (2006). Integrin-regulated FAK-Src signaling in normal and cancer cells.
  192. (1998). Integrinmediated signals regulated by members of the rho family of GTPases.
  193. (2009). Integrins and cell-fate determination.
  194. (1996). Integrins and disease.
  195. (1996). Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors.
  196. (2009). Integrins in cell migration--the actin connection.
  197. (2009). Integrins in immunity.
  198. (1989). Integrins isolated from Rous sarcoma virus-transformed chicken embryo fibroblasts.
  199. (2002). Integrins: bidirectional, allosteric signaling machines.
  200. (2002). Interaction of Axl receptor tyrosine kinase with C1-TEN, a novel C1 domain-containing protein with homology to tensin.
  201. (2006). Interaction of deleted in liver cancer 1 with tensin2 in caveolae and implications in tumor suppression.
  202. (1985). Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts.
  203. (1986). Interactions of migrating neural crest cells with fibronectin.
  204. (1994). Interactions of tensin with actin and identification of its three distinct actin-binding domains.
  205. (1999). Interplay between Rac and Rho in the control of substrate contact dynamics.
  206. (2004). Intersubunit signal transmission in integrins by a receptor-like interaction with a pull spring.
  207. (2000). Intestinal restitution: progression of actin cytoskeleton rearrangements and integrin function in a model of epithelial wound healing.
  208. (1982). Intracellular and extracellular components involved in the formation of ventral surfaces of fibroblasts.
  209. (2005). Intrinsically unstructured proteins and their functions.
  210. (2002). Intrinsically unstructured proteins.
  211. (2009). Invadopodia: specialized tumor cell structures for the focal degradation of the extracellular matrix.
  212. (2006). is associated with aggressive tumor behavior in human hepatocellular carcinoma.
  213. (2008). Kindlin-2 controls bidirectional signaling of integrins.
  214. (2008). Kindlin-3 is essential for integrin activation and platelet aggregation.
  215. (2009). Leukocyte adhesion deficiency syndrome: a controversy solved.
  216. (2009). Linking integrin conformation to function.
  217. (2008). Local structural preferences of calpastatin, the intrinsically unstructured protein inhibitor of calpain.
  218. (1991). Localization of paxillin, a focal adhesion protein, to smooth muscle dense plaques, and the myotendinous and neuromuscular junctions of skeletal muscle.
  219. (2003). Localized cdc42 activation, detected using a novel assay, mediates microtubule organizing center positioning in endothelial cells in response to fluid shear stress.
  220. (2000). Localized Rac activation dynamics visualized in living cells.
  221. (2008). Loss of Kindlin-1 causes skin atrophy and lethal neonatal intestinal epithelial dysfunction.
  222. (1983). Lymphocyte migration into threedimensional collagen matrices: a quantitative study.
  223. (1990). Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor alpha 4 beta 1.
  224. (1997). Lysophosphatidic acid and microtubule-destabilizing agents stimulate fibronectin matrix assembly through Rhodependent actin stress fiber formation and cell contraction.
  225. (2005). Mapping and consensus sequence identification for multiple vinculin binding sites within the talin rod.
  226. (1996). Mapping of actin, vinculin, and integrin binding domains suggests a direct role of tensin in actin-membrane association.
  227. (2001). Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior.
  228. (2002). Matrix metalloproteinase inhibitors and cancer: trials and tribulations.
  229. (1993). Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment strength.
  230. (2003). Mechanism of filopodia initiation by reorganization of a dendritic network.
  231. (1981). Mechanism of retraction of the trailing edge during fibroblast movement.
  232. (2005). Mechanisms of cancer cell invasion.
  233. (2009). Mechanisms that regulate adaptor binding to beta-integrin cytoplasmic tails.
  234. (2006). Membrane binding domains.
  235. (1983). Membrane insertion at the leading edge of motile fibroblasts.
  236. (2009). Metastasis: from dissemination to organ-specific colonization.
  237. (1999). Microtubule targeting of substrate contacts promotes their relaxation and dissociation.
  238. (2005). Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase.
  239. (2004). Migration of epidermal keratinocytes: mechanisms, regulation, and biological significance.
  240. (1997). Migration of highly aggressive MV3 melanoma cells in 3-dimensional collagen lattices results in local matrix reorganization and shedding of alpha2 and beta1 integrins and CD44.
  241. (1987). Mobility of filamentous actin in living cytoplasm.
  242. (1982). Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery.
  243. (2007). Modeling tissue morphogenesis and cancer in 3D.
  244. (2010). modulates cell contractility in 3D collagen gels through the RhoGAP DLC1.
  245. (1996). Modulation of cell migration by integrin-mediated cytoskeletal linkages and ligand-binding affinity.
  246. Modulation of cell surface fibronectin assembly sites by lysophosphatidic acid.
  247. (2002). Molecular biology of the cell, 4th edn
  248. (2000). Molecular characterization of human tensin.
  249. (1994). Molecular cloning of chick cardiac muscle tensin. Full-length cDNA sequence, expression, and characterization.
  250. (1995). Molecular cloning, expression, and mapping of the high affinity actin-capping domain of chicken cardiac tensin.
  251. (2009). Molecular dissection of the ILK-PINCH-parvin triad reveals a fundamental role for the ILK kinase domain in the late stages of focaladhesion maturation.
  252. Molecular diversity of cell-matrix adhesions.
  253. (2005). Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin.
  254. (1999). Morphological changes and detachment of adherent cells induced by p122, a GTPase-activating protein for Rho.
  255. (2006). Multiple functions of the integrin alpha6beta4 in epidermal homeostasis and tumorigenesis.
  256. (1996). Myosin drives retrograde F-actin flow in neuronal growth cones.
  257. (2007). Myosin II and mechanotransduction: a balancing act.
  258. (2007). Myosin IIA regulates cell motility and actomyosin-microtubule crosstalk.
  259. myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo.
  260. (1998). Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization.
  261. (2001). Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts.
  262. (2001). Nephrocystin interacts with Pyk2, p130(Cas), and tensin and triggers phosphorylation of Pyk2.
  263. (2008). New insights into form and function of fibronectin splice variants.
  264. (2003). New insights into the structural basis of integrin activation.
  265. (2006). Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
  266. (2005). Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells.
  267. (1997). Nuclear-cytoplasmic shuttling of the focal contact protein, zyxin: a potential mechanism for communication between sites of cell adhesion and the nucleus.
  268. (2008). On the Rho'd: the regulation of membrane protrusions by Rho-GTPases.
  269. (2007). Oncogenic inhibition by a deleted in liver cancer gene requires cooperation between tensin binding and Rho-specific GTPase-activating protein activities.
  270. (1996). p130CAS forms a signaling complex with the adapter protein CRKL in hematopoietic cells transformed by the BCR/ABL oncogene.
  271. (1997). p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin.
  272. (2007). Pathophysiology of acute wound healing.
  273. (2002). Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells.
  274. (2003). Phosphatases in cell-matrix adhesion and migration.
  275. (1996). Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase).
  276. (1998). Physical and biochemical regulation of integrin release during rear detachment of migrating cells.
  277. (2000). Physical state of the extracellular matrix regulates the structure and molecular composition of cell-matrix adhesions.
  278. (2003). PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival.
  279. (1996). Plateletderived growth factor-induced formation of tensin and phosphoinositide 3-kinase complexes.
  280. (2008). Podosome-type adhesions and focal adhesions, so alike yet so different.
  281. (2005). Podosomes at a glance.
  282. (2003). Podosomes display actin turnover and dynamic self-organization in osteoclasts expressing actingreen fluorescent protein.
  283. (2007). Polymerizing actin fibers position integrins primed to probe for adhesion sites.
  284. (1995). pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk.
  285. (2001). pp60(c-src) and related tyrosine kinases: a role in the assembly and reorganization of matrix adhesions.
  286. (2007). Preparation of extracellular matrices produced by cultured and primary fibroblasts.
  287. (1991). Presence of an SH2 domain in the actin-binding protein tensin.
  288. (2006). Probing the integrin-actin linkage using high-resolution protein velocity mapping.
  289. (2008). Prognostic relevance of Tensin4 expression in human gastric cancer.
  290. (1997). Progressive kidney degeneration in mice lacking tensin.
  291. (1999). Proteolysis and cell migration: creating a path?
  292. (2009). Proteolytic interstitial cell migration: a five-step process.
  293. (1987). Purification of a vinculin binding protein from smooth muscle.
  294. (2008). Rab-coupling protein coordinates recycling of alpha5beta1 integrin and EGFR1 to promote cell migration in 3D microenvironments.
  295. (2008). Rac activation and inactivation control plasticity of tumor cell movement.
  296. (2003). Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis.
  297. (2009). Rac and Rho driving tumor invasion: who's at the wheel?
  298. (1999). Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior.
  299. (2009). Random versus directionally persistent cell migration.
  300. (2008). Rapid leukocyte migration by integrin-independent flowing and squeezing.
  301. (1995). Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice.
  302. (2005). Regulating cell migration: calpains make the cut.
  303. (2008). Regulating the conversion between rounded and elongated modes of cancer cell movement.
  304. (2008). Regulation of actin assembly associated with protrusion and adhesion in cell migration.
  305. (2007). Regulation of actin filament assembly by Arp2/3 complex and formins.
  306. (1996). Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase.
  307. (2007). Regulation of cell adhesion by affinity and conformational unbending of alpha4beta1 integrin.
  308. (1997). Regulation of cell migration by the calcium-dependent protease calpain.
  309. (1997). Regulation of cell motility by mitogen-activated protein kinase.
  310. (1999). Regulation of cell movement is mediated by stretch-activated calcium channels.
  311. Regulation of fibronectin matrix assembly and capillary morphogenesis in endothelial cells by Rho family GTPases.
  312. (2002). Regulation of focal complex composition and disassembly by the calcium-dependent protease calpain.
  313. (2004). Regulation of integrin-mediated cellular responses through assembly of a CAS/Crk scaffold.
  314. (2005). Regulation of microtubules in cell migration.
  315. (1996). Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase).
  316. (2007). Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells.
  317. (2003). Regulation of tensin-promoted cell migration by its focal adhesion binding and Src homology domain 2.
  318. (2002). Regulatory role for SRC and phosphatidylinositol 3-kinase in initiation of fibronectin matrix assembly.
  319. (1982). Reorganization of cytoskeletal and contractile elements during transition of human monocytes into adherent macrophages.
  320. (2004). Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors.
  321. (2004). Restoration of DLC-1 gene expression induces apoptosis and inhibits both cell growth and tumorigenicity in human hepatocellular carcinoma cells.
  322. (1994). Reversible unfolding of fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin.
  323. (2007). Rho activation at a glance.
  324. (2005). Rho GTPase-activating protein deleted in liver cancer suppresses cell proliferation and invasion in hepatocellular carcinoma.
  325. (2003). Rho GTPase-activating proteins in cell regulation.
  326. (2001). Rho GTPases and cell migration.
  327. (1999). Rho GTPases control polarity, protrusion, and adhesion during cell movement.
  328. (2004). Rho GTPases have diverse effects on the organization of the actin filament system.
  329. (2004). Rho signalling at a glance.
  330. (2004). Rho-family GTPases: it's not only Rac and Rho (and I like it).
  331. (1998). Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly.
  332. (1996). Rho-stimulated contractility drives the formation of stress fibers and focal adhesions.
  333. (1995). Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.
  334. (2001). RhoA is required for monocyte tail retraction during transendothelial migration.
  335. (1982). Role of fibronectin in collagen deposition: Fab' to the gelatin-binding domain of fibronectin inhibits both fibronectin and collagen organization in fibroblast extracellular matrix.
  336. (1992). Role of integrin alpha 4 beta 7/alpha 4 beta P in lymphocyte adherence to fibronectin and VCAM-1 and in homotypic cell clustering.
  337. (1999). Role of integrins in cancer: survey of expression patterns.
  338. (2002). Sensing the environment: a historical perspective on integrin signal transduction.
  339. (2004). Shear-dependent capping of L-selectin and P-selectin glycoprotein ligand 1 by E-selectin signals activation of high-avidity beta2-integrin on neutrophils.
  340. (2009). Signal co-operation between integrins and other receptor systems.
  341. (1999). Signaling through focal adhesion kinase.
  342. (1998). SMART, a simple modular architecture research tool: identification of signaling domains.
  343. (2007). SnapShot: Rho family GTPases.
  344. (2002). Spatiotemporal regulation of moesin phosphorylation and rear release by Rho and serine/threonine phosphatase during neutrophil migration.
  345. (1999). Src family kinases are required for integrin but not PDGFR signal transduction.
  346. (2009). START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region.
  347. (2005). Stimulatory effects of a three-dimensional microenvironment on cell-mediated fibronectin fibrillogenesis.
  348. (2006). Stress fibers are generated by two distinct actin assembly mechanisms in motile cells.
  349. (2003). Structural and compositional analysis of the keratinocyte migration track.
  350. (2004). Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics.
  351. (2000). Structural basis of collagen recognition by integrin alpha2beta1.
  352. (2007). Structural basis of integrin activation by talin.
  353. (2003). Structural determinants of integrin recognition by talin.
  354. (1996). Structural studies of p21Waf1/Cip1/Sdi1 in the free and Cdk2-bound state: conformational disorder mediates binding diversity.
  355. (2007). Structure and mechanics of integrin-based cell adhesion.
  356. Structure of human platelet membrane glycoproteins IIb and IIIa as determined by electron microscopy.
  357. (2007). Structure of the PTB domain of tensin1 and a model for its recruitment to fibrillar adhesions.
  358. (1986). Studies on proteins that co-purify with smooth muscle vinculin: identification of immunologically related species in focal adhesions of nonmuscle and Z-lines of muscle cells.
  359. (2002). Studying actin-dependent processes in tissue culture.
  360. (2007). Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix.
  361. (1953). Syndrome of congenital hemihypertrophy, shortness of stature, and elevated urinary gonadotropins.
  362. (1998). Synergy between actin depolymerizing factor/cofilin and profilin in increasing actin filament turnover.
  363. (2001). Taking cellmatrix adhesions to the third dimension.
  364. (1996). Talin and vinculin play distinct roles in filopodial motility in the neuronal growth cone.
  365. (2003). Talin binding to integrin beta tails: a final common step in integrin activation.
  366. (2004). Talin controls integrin activation.
  367. Talin depletion reveals independence of initial cell spreading from integrin activation and traction.
  368. (1995). Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype.
  369. (1996). Targeted mutations in cell adhesion genes: what have we learned from them?
  370. (2004). Tensin is potentially involved in extracellular matrix production in mesangial cells.
  371. (2004). Tensin stabilizes integrin adhesive contacts in Drosophila.
  372. (2002). Tensin1 and a previously undocumented family member, tensin2, positively regulate cell migration.
  373. (2009). Tensin3 is a negative regulator of cell migration and all four Tensin family members are downregulated in human kidney cancer.
  374. (2006). Tensin3 is a novel thyroid-specific gene.
  375. (2000). The biology of cell locomotion within threedimensional extracellular matrix.
  376. (2005). The cancer chemopreventive agent resveratrol induces tensin, a cell-matrix adhesion protein with signaling and antitumor activities.
  377. (1998). The catalytic activity of Src is dispensable for translocation to focal adhesions but controls the turnover of these structures during cell motility.
  378. (2000). The collection of the motile population of cells from a living tumor.
  379. (1993). The extracellular matrix as a cell survival factor.
  380. (1987). The final stages of wing development in Drosophila melanogaster.
  381. (2008). The genetic aetiology of Silver-Russell syndrome.
  382. (2002). The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix.
  383. (2007). The integrin-growth factor receptor duet.
  384. (1998). The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments.
  385. (1971). The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
  386. (2007). The matrix corroded: podosomes and invadopodia in extracellular matrix degradation.
  387. (1996). The N-terminal domains of tensin and auxilin are phosphatase homologues.
  388. (1996). The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton.
  389. (2007). The phosphotyrosineindependent interaction of DLC-1 and the SH2 domain of cten regulates focal adhesion localization and growth suppression activity of DLC-1.
  390. (2008). The PTB domain of tensin: NMR solution structure and phosphoinositides binding studies.
  391. (2004). The RAB25 small GTPase determines aggressiveness of ovarian and breast cancers.
  392. (2005). The Ras superfamily at a glance.
  393. (2003). The RGD story: a personal account.
  394. (2005). The Rho family GTPase Rif induces filopodia through mDia2.
  395. (2005). The RhoGAP protein DLC-1 functions as a metastasis suppressor in breast cancer cells.
  396. (2009). The role of formins in filopodia formation.
  397. (2008). The role of integrin binding sites in fibronectin matrix assembly in vivo.
  398. (1994). The short amino acid sequence ProHis-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function.
  399. (1992). The small GTP-binding protein rac regulates growth factor-induced membrane ruffling.
  400. (1996). The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase.
  401. (2006). The structure and regulation of vinculin.
  402. (2009). The structure of the integrin alphaIIbbeta3 transmembrane complex explains integrin transmembrane signalling.
  403. (2009). The Tensin-3 protein, including its SH2 domain, is phosphorylated by Src and contributes to tumorigenesis and metastasis.
  404. (2005). Three-dimensional EM structure of the ectodomain of integrin {alpha}V{beta}3 in a complex with fibronectin.
  405. (2007). Three-dimensional microenvironments modulate fibroblast signaling responses.
  406. (2007). to promote invasive migration in 3D microenvironments.
  407. (1996). Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich syndrome.
  408. (1995). Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions.
  409. (2009). Up-regulation of Cterminal tensin-like molecule promotes the tumorigenicity of colon cancer through betacatenin.
  410. (1998). WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.