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Streptococcus pneumoniae: involvement of neuraminidase, autolysin and superoxide dismutase in respiratory tract infections

By Sonia Da Cruz Manco

Abstract

This study focuses on the Streptococcus pneumoniae virulence factors\ud neuraminidases A and B, autolysin and superoxide dismutase, with the aim of\ud improved understanding of their roles in the pneumococcal infections.\ud Understanding the pathogenesis of pneumococcal infection is important because\ud this bacterium is one of the most common bacterial causes of human disease and\ud death and because of the emergence of multidrug-resistant strains and issues with\ud the current vaccines.\ud Using a murine model of bronchopneumonia it was shown that NanA and\ud NanB have involvement in colonisation of the upper respiratory tract and in\ud systemic spread. Lack of either of these proteins enhanced bacterial clearance in\ud the nasopharynx, lungs and blood. Furthermore, this study demonstrated distinct\ud roles for each of the neuraminidases, but also suggests synergistic action.\ud Infection data clearly show that the absence of one neuraminidase is not\ud compensated by the presence of the other. This study identified, for the first time,\ud a role for NanB in pathogenesis and in multiplication at mucosal sites.\ud The major S. pneumoniae autolysin LytA has been implicated in\ud pathogenicity by releasing virulence factors and components of the cell wall. In this\ud study, the avirulence of a pneumococcal autolysin-deficient mutant was confirmed.\ud Moreover, the results show that early after infection, LytA is crucial to\ud pneumococcal survival and growth, both in the upper and lower respiratory tract.\ud The major pneumococcal enzyme for detoxifying superoxide is MnSOD,\ud encoded by sodA. This study showed that absence of sodA impaired survival in\ud the respiratory tract, particularly early after infection, but its absence was not\ud lethal, suggesting that other mechanisms are involved in superoxide detoxification

Publisher: University of Leicester
Year: 2010
OAI identifier: oai:lra.le.ac.uk:2381/7948

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Citations

  1. (1995). 2nd Ed.
  2. (2005). 3D structure of choline binding proteins. Paper presented at 7th European Meeting on the Molecular Biology of Pneumococcus,
  3. (1993). 4-guanidino-2,4-influenza A and B viruses in vitro.
  4. (1990). 4-methylumbelliferyl-alpha-glycosides of partially O-acetylated Nacetylneuraminic acids as substrates of bacterial and viral sialidases.
  5. (1992). A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism.
  6. (1982). A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis.
  7. (1994). A neuraminidase from Streptococcus pneumoniae has the features of a surface protein.
  8. (1964). A new synthetic medium for Diplococcus pneumoniae, and its use for the study of reciprocal transformations at the amia locus.
  9. (1996). A novel nickelcontaining superoxide dismutase from Streptomyces spp.
  10. (2001). A novel solenoid fold in the cell wall anchoring domain of the pneumococcal virulence factor LytA.
  11. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
  12. (2006). A review of vaccine research and development: Meningococcal disease.
  13. (1997). A role for pneumolysin but not neuraminidase in the hearing loss and cochlear damage induced by experimental pneumococcal meningitis in guinea pigs.
  14. (1994). A role in cellbinding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae.
  15. (2000). Achievements and challenges of sialic acid research.
  16. (1975). Action of neuraminidases isolated from Diplococcus pneumoniae and Clostridium perfringens on the viscosity and elastic properties of bronchial secretions.
  17. (2006). Acute respiratory infections (ARI) research in the Gambia. Seminar held at the University of Leicester,
  18. (1985). Acute respiratory infections in under-fives: 15 million deaths a year.
  19. (2000). Additive attenuation of virulence of Streptococcus pneumoniae by mutation of the genes encoding pneumolysin and other putative pneumococcal virulence proteins.
  20. (1981). Adhesion of Streptococcus pneumoniae to human pharyngeal epithelial cells in vitro: Differences in adhesive capacity among strains isolated from subjects with otitis media, septicemia, or meningitis or from healthy carriers.
  21. (2004). An important role for polymeric Ig receptor-mediated transport of IgA in protection against Streptococcus pneumoniae nasopharyngeal carriage.
  22. and biochemical characterization of the pneumococcal bacteriophage cp-1 lysin.
  23. (2008). Animal models of Streptococcus pneumoniae diseases.
  24. (2002). Antibiotic tolerance in pneumococci.
  25. (2003). Antibody-enhanced pneumococcal adherence requires
  26. (1996). Applications for green fluorescent protein (GFP) in the study of hostpathogen interactions.
  27. (2004). Are respiratory enzymes the primary sources of intracellular hydrogen peroxide?
  28. (2006). Attachment of capsular polysaccharide to the cell wall of Streptococcus pneumoniae type 2 is required for invasive disease.
  29. (2004). Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples.
  30. (1994). Autolysis-defective mutant of Staphylococcus aureus: Pathological considerations, genetic mapping and electron microscopic studies.
  31. (1999). Bacillus subtilis 168 gene lytF encodes a gamma-D-glutamate-meso-diaminopimelate muropeptidase 145
  32. (1997). Bacterial cell surface carbohydrates: Structure and assembly.
  33. (1997). Bacterial copper- and zinc-cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis.
  34. (1943). Bacterial morphology as shown by the electron microscope: VI. Capsule, cell-wall and inner protoplasm of pneumococcus, type III.
  35. (2006). Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production.
  36. (1992). Bacterial sialidases: roles in pathogenicity and nutrition.
  37. (1998). Balance between endogenous superoxide stress and antioxidant defenses.
  38. (1996). Binding between outer membrane proteins of nontypeable Haemophilus influenzae and human nasopharyngeal mucin.
  39. (2006). Biochemical and physical properties of green fluorescent protein in Green fluorescent protein: Properties, applications and protocols.
  40. (1987). Biological role of the pneumococcal amidase: cloning of the lytA gene in Streptococcus pneumoniae.
  41. (2000). Biological roles of two new murein hydrolases of Streptococcus pneumoniae representing examples of module shuffling.
  42. (2005). Black and WHO Child Health Epidemiology Reference Group.
  43. (2000). C3 as substrate for adhesion of Streptococcus pneumoniae.
  44. (1982). Chemistry, metabolism, and biological functions of sialic acids.
  45. (1996). Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli.
  46. (1985). Cloning and expression of the pneumococcal autolysin gene in Escherichia coli.
  47. (1988). Cloning and expression of the pneumococcal neuraminidase gene in Escherichia coli.
  48. (1991). Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis.
  49. (1992). Comparative efficacy of autolysin and pneumolysin as immunogens protecting mice against infection by streptococcus pneumoniae.
  50. (1988). Comparative efficacy of pneumococcal neuraminidase and pneumolysin as immunogens protective against Streptococcus pneumoniae.
  51. (2001). Comparison of structural changes of cell surface carbohydrates in the eustachian tube epithelium of chinchillas infected with a Streptococcus pneumoniae neuraminidase-deficient mutant or its isogenic parent strain.
  52. (1997). Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases.
  53. (1972). Competence of pneumococcal isolates and bacterial transformations in man.
  54. (2005). Competenceprogrammed predation of noncompetent cells in the human pathogen Streptococcus pneumoniae: Genetic requirements.
  55. (1991). Complement activation and antibody binding by pneumolysin via a region of the toxin homologous to a human acute-phase protein.
  56. (2003). Complement resistance mechanisms of streptococci.
  57. (2001). Complete genome sequence of a virulent isolate of Streptococcus pneumoniae.
  58. (2004). Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related bacillus species.
  59. (1994). Conserved patterns in the Cu/Zn superoxide dismutase family.
  60. (1989). Conserved sequences in bacterial and viral sialidases.
  61. (1989). Contribution of autolysin to virulence of Streptococcus pneumoniae.
  62. (2002). Contribution of choline-binding proteins to cell surface properties of Streptococcus pneumoniae.
  63. (2001). Contribution of Mn-cofactored superoxide dismutase (SodA) to the virulence of Streptococcus agalactiae.
  64. (1997). Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae.
  65. (2002). Contribution of pneumolysin and autolysin to the pathogenesis of experimental pneumococcal endophthalmitis.
  66. (1996). Crystal structure of the Aequorea victoria green fluorescent protein.
  67. (2008). Crystal structure of the NanB sialidase from Streptococcus pneumoniae.
  68. (2002). Decreased virulence of a pneumolysin-deficient strain of Streptococcus pneumoniae in murine meningitis.
  69. (1974). Defective superoxide production by granulocytes from patients with chronic granulomatous disease. The New England
  70. (2006). Deglycosylation of human glycoconjugates by the sequential activities of exoglycosidases expressed by Streptococcus pneumoniae.
  71. (1995). Dual function of pneumolysin in the early pathogenesis of murine pneumococcal pneumonia.
  72. (2002). Dynamics of nasopharyngeal colonization by potential respiratory pathogens.
  73. (2007). Effect of hydrogen peroxide production and the fenton reaction on membrane composition of Streptococcus pneumoniae.
  74. (1983). Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae.
  75. (1992). Effect of insertional inactivation of the genes encoding pneumolysin and autolysin on the virulence of Streptococcus pneumoniae type 3.
  76. (2006). Effect of introduction of the pneumococcal conjugate vaccine on drug-resistant Streptococcus pneumoniae. The New England
  77. (2002). Effect of neuraminidase on receptor-mediated adherence of Streptococcus pneumoniae to chinchilla tracheal epithelium.
  78. (2006). Effect of pneumococcal conjugate vaccine on nasopharyngeal bacterial colonization during acute otitis media.
  79. (2004). Effect of the 7-valent pneumococcal conjugate vaccine on nasopharyngeal colonization by Streptococcus pneumoniae in the first 2 years of life.
  80. (1994). Effects of H2O2-producing lactobacilli on Neisseria gonorrhoeae growth and catalase activity.
  81. (2000). Efficacy, safety and immunogenicity of heptavalent pneumococcal conjugate vaccine in children. Northern California kaiser permanente vaccine study center group. The Pediatric Infectious Disease
  82. (1999). Emergence of vancomycin tolerance in Streptococcus pneumoniae.
  83. (2005). Epidemiological differences among pneumococcal serotypes.
  84. (1995). Epidemiology of nasopharyngeal colonization with nontypeable Haemophilus influenzae in the first 2 years of life.
  85. (1994). Escherichia coli expresses a copper- and zinccontaining superoxide dismutase.
  86. (2004). Essential role for the p40 subunit of interleukin-12 in neutrophil-mediated early host defense against pulmonary infection with Streptococcus pneumoniae: Involvement of interferon-gamma.
  87. (1997). Establishment of a Streptococcus pneumoniae nasopharyngeal colonization model in adult mice.
  88. (2000). Evaluation of the virulence of a Streptococcus pneumoniae neuraminidase-deficient mutant in nasopharyngeal colonization and development of otitis media in the chinchilla model.
  89. (1997). Evidence for autolysinmediated primary attachment of Staphylococcus epidermidis to a polystyrene surface.
  90. (1975). Experimental pneumococcal meningitis: effects of neuraminidase and other pneumococcal constituents on cerebrospinal fluid in the intact dog.
  91. (2000). Expression and role of superoxide dismutases (SOD) in pathogenic bacteria.
  92. (1998). Extracellular superoxide dismutase from Streptococcus pyogenes type 12 strain is manganesedependent.
  93. (1962). Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan,
  94. (2003). Factors contributing to hydrogen peroxide resistance in Streptococcus pneumoniae include pyruvate oxidase (SpxB) and avoidance of the toxic effects of the Fenton reaction.
  95. (2005). for Disease Control and Prevention.
  96. (2003). From nose to lung: The regulation behind Streptococcus pneumoniae virulence factors.
  97. (1999). Fundamental aspects of reactive oxygen species, or what's the matter with oxygen?
  98. Generation of a superoxide dismutase (SOD)-deficient mutant of Campylobacter coli: Evidence for the significance of SOD in campylobacter survival and colonization.
  99. (1994). Genetic, enzymatic, and pathogenic studies of the iron superoxide dismutase of Campylobacter jejuni.
  100. (2001). Genome of the bacterium Streptococcus pneumoniae strain R6.
  101. (2004). Glycolytic enzymes associated with the cell surface of Streptococcus pneumoniae are antigenic in humans and elicit protective immune responses in the mouse. Clinical and Experimental Immunology 138, (2)
  102. (1994). Green fluorescent protein as a marker for gene expression.
  103. (1995). Green fluorescent protein as a new expression marker in mycobacteria.
  104. (2001). Group A streptococci bind to mucin and human pharyngeal cells through sialic acid-containing receptors.
  105. (2008). Growth of Streptococcus pneumoniae on human glycoconjugates is dependent upon the sequential activity of bacterial exoglycosidases.
  106. (1999). Health Organisation.
  107. (2000). Host cellular immune response to pneumococcal lung infection in mice.
  108. (1994). Human milk secretory IgA antibody to nontypeable Haemophilus influenzae: Possible protective effects against nasopharyngeal colonization.
  109. (2006). Identification of amino acids essential for catalytic activity of pneumococcal neuraminidase A.
  110. (1983). Identification of an active disaccharide unit of a glycoconjugate receptor for pneumococci attaching to human pharyngeal epithelial cells.
  111. (1993). Identification of hydrogen peroxide as a Streptococcus pneumoniae toxin for rat alveolar epithelial cells.
  112. (2001). Identification of the teichoic acid phosphorylcholine esterase in Streptococcus pneumoniae.
  113. (1996). Identification, cloning, and sequencing of the immunoglobulin A1 protease gene of Streptococcus pneumoniae.
  114. (2005). Illustration of pneumococcal polysaccharide capsule during adherence and invasion of epithelial cells.
  115. Immune responses to specific antigens of Streptococcus pneumoniae and Moraxella catarrhalis in the respiratory tract.
  116. (2000). Immunization of humans with recombinant pneumococcal surface protein A (rPspA) elicits antibodies that passively protect mice from fatal infection with Streptococcus pneumoniae bearing heterologous PspA.
  117. (1994). Immunization of mice with pneumolysin toxoid confers a significant degree of protection against at least nine serotypes of Streptococcus pneumoniae.
  118. (2001). Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection.
  119. (2005). Immunization with recombinant Streptococcus pneumoniae neuraminidase NanA protects chinchillas against nasopharyngeal colonization.
  120. (2005). Implications of physiological studies based on genomic sequences: Streptococcus pneumoniae TIGR4 synthesizes a functional LytC lysozyme.
  121. (1995). Improved green fluorescence.
  122. (2001). In vitro resistance of Burkholderia cepacia complex isolates to reactive oxygen species in relation to catalase and superoxide dismutase production.
  123. (2002). Inactivation of the Moraxella catarrhalis superoxide dismutase SodA induces constitutive expression of iron-repressible outer membrane proteins.
  124. (1999). Increased carriage of resistant nonpneumococcal alpha-hemolytic streptococci after antibiotic therapy.
  125. (1971). Increased resistance to penicillin of pneumococci isolated from man. The New England
  126. (1994). Influenza virus neuraminidase: structure, antibodies, and inhibitors.
  127. (2000). Inhibition of OM pathogens by alpha-hemolytic streptococci from healthy children, children with SOM and children with rAOM.
  128. (2000). Inhibitory and bactericidal effects of hydrogen peroxide production by Streptococcus pneumoniae on other inhabitants of the upper respiratory tract.
  129. (1996). Insertional inactivation of Streptococcus pyogenes sod suggests that prtF is regulated in response to a superoxide signal.
  130. (1974). Intermolecular energy transfer in the bioluminescent system of Aequorea.
  131. (2000). Intranasal immunization of mice with a mixture of the pneumococcal proteins PsaA and PspA is highly protective against nasopharyngeal carriage of Streptococcus pneumoniae.
  132. (1974). Isolation of a new copper-containing superoxide dismutase bacteriocuprein.
  133. (1992). Limited tryptic cleavage of complement factor H abrogates recognition of sialic acid-containing surfaces by the alternative pathway of complement.
  134. (2005). Low-dose aerosol model of pneumococcal pneumonia in the mouse: utility for evaluation of antimicrobial efficacy.
  135. (1999). LytB, a novel pneumococcal murein hydrolase essential for cell separation.
  136. (1988). Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc beta 1-4Gal found in some glycolipids.
  137. (2000). Mechanisms of antibiotic resistance and tolerance in Streptococcus pneumoniae.
  138. (2004). Method for inducing experimental pneumococcal meningitis in outbred mice.
  139. (2004). Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae.
  140. (2001). Microbiological and inflammatory factors associated with development of pneumococcal pneumonia.
  141. (2004). Microbiology and drug resistance mechanisms of fully resistant pathogens.
  142. (1997). Molecular analysis of putative pneumococcal virulence proteins.
  143. (1993). Molecular analysis of the pathogenicity of Streptococcus pneumoniae: The role of pneumococcal proteins.
  144. (2004). Molecular analysis of the psa permease complex of Streptococcus pneumoniae.
  145. (1999). Molecular and cellular biology of pneumococcal infection.
  146. (2001). Molecular characterization of the pneumococcal teichoic acid phosphorylcholine esterase.
  147. (1989). Molecular cloning: A laboratory
  148. (1988). Molecular evolution of lytic enzymes of Streptococcus pneumoniae and its bacteriophages.
  149. (1992). Molecular localization of variable and conserved regions of PspA and identification of additional PspA homologous sequences in Streptococcus pneumoniae.
  150. (1992). Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin.
  151. (1992). Mucin degradation in the human colon: production of sialidase, sialate Oacetylesterase, N-acetylneuraminate lyase, arylesterase, and glycosulfatase activities by strains of fecal bacteria.
  152. (2001). Mucinases and sialidases: Their role in the pathogenesis of sexually transmitted infections in the female genital tract.
  153. (2005). Mucosal immunisation with novel Streptococcus pneumoniae protein antigens enhances bacterial clearance in an acute mouse lung infection model.
  154. (2005). NanA, a neuraminidase from Streptococcus pneumoniae, shows high levels of sequence diversity, at least in part through recombination with Streptococcus oralis.
  155. (2005). Nasopharyngeal colonization with heptavalent pneumococcal conjugate vaccine serotypes of Streptococcus pneumoniae with prolonged vaccine dosing intervals. The Pediatric Infectious Disease
  156. (1967). Neuraminidase activities of clinical isolates of Diplococcus pneumoniae.
  157. (1971). Neuraminidase activity in bacterial meningitis.
  158. (2002). Neuraminidase expressed by Streptococcus pneumoniae desialylates the lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae: A paradigm for interbacterial competition among pathogens of the human respiratory tract.
  159. (2000). Neuraminidases (sialidases) from bacterial origin.
  160. (2004). New developments with Streptococcus pneumoniae.
  161. (1983). Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning.
  162. (1975). Novel type of murein transglycosylase in Escherichia coli.
  163. (2003). Novel vaccine strategies with protein antigens of Streptococcus pneumoniae.
  164. (1992). Nucleotide sequence of Streptococcus mutans superoxide dismutase gene and isolation of insertion mutants.
  165. (2009). OperonDB: A comprehensive database of predicted operons in microbial genomes.
  166. (2001). Out of the iron age: New insights into the critical role of manganese homeostasis in bacteria.
  167. (2002). Oxidative stress tolerance is manganese (Mn(2+)) regulated in Streptococcus gordonii.
  168. (1998). Oxygen toxicity: A radical explanation.
  169. (1995). Pathogenesis of pneumococcal infection.
  170. (1995). Peptide permeases from Streptococcus pneumoniae affect adherence to eukaryotic cells.
  171. (1998). Periplasmic superoxide dismutase in meningococcal pathogenicity.
  172. (2000). Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties.
  173. (2004). Phase variable desialylation of host proteins that bind to Streptococcus pneumoniae in vivo and protect the airway.
  174. (1994). Phase variation in pneumococcal opacity: Relationship between colonial morphology and nasopharyngeal colonization.
  175. (1989). Physicochemical properties of bacterial surfaces.
  176. (2003). Pneumococcal behavior and host responses during bronchopneumonia are affected differently by the cytolytic and complement-activating activities of pneumolysin.
  177. (2004). Pneumococcal disease: pathogenesis, treatment, and prevention.
  178. (1998). Pneumococcal diversity: Considerations for new vaccine strategies with emphasis on pneumococcal surface protein A (PspA).
  179. (2004). Pneumococcal infections in www.eMedicine.com.
  180. (2006). Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis.
  181. (2005). Pneumococcal vaccination.
  182. (1999). Pneumococcal vaccine: An update.
  183. Pneumococcal vaccines: An update on current strategies.
  184. (2001). Pneumococcal virulence factors: Structure and function.
  185. (2003). Pneumococcal zinc metalloproteinase ZmpC cleaves human matrix metalloproteinase 9 and is a virulence factor in experimental pneumonia.
  186. (2005). Pneumococcus forks out...
  187. (2004). Pneumococcus virulence factor sialidase: A new direction in neuro-AIDS research?
  188. (1988). Possible role of neuraminidase in activated T cells in the recognition of allogeneic Ia.
  189. (1997). Prevention of pneumococcal disease: Recommendations of the advisory committee on immunization practice
  190. (1992). Primary structure of the Aequorea victoria green-fluorescent protein.
  191. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
  192. (2000). Progress toward the development of a vaccine to prevent Moraxella (branhamella) catarrhalis infections.
  193. (1979). Properties of extracellular neuraminidase produced by group A streptococcus.
  194. (1995). Pseudomonas aeruginosa sodA and sodB mutants defective in manganese- and ironcofactored superoxide dismutase activity demonstrate the importance of the iron-cofactored form in aerobic metabolism.
  195. (1996). PspA, a protection-eliciting pneumococcal protein: Immunogenicity of isolated native PspA in mice.
  196. (2001). PspC, a pneumococcal surface protein, binds human factor H.
  197. (2002). Purification and polar localization of pneumococcal LytB, a putative endo-beta-Nacetylglucosaminidase: The chain-dispersing murein hydrolase.
  198. (1989). Purification and properties of Streptococcus pneumoniae neuraminidase.
  199. (2004). Pyruvate oxidase is a determinant of Avery's rough morphology.
  200. (1996). Pyruvate oxidase, as a determinant of virulence in Streptococcus pneumoniae.
  201. (2000). Questions about the behaviour of bacterial pathogens
  202. (2006). Ramirez and Grupo de Estudo Português de Bactérias Patogénicas Respiratórias.
  203. (2007). Rapid, accurate, computational discovery of Rho-independent transcription terminators illuminates their relationship to DNA uptake.
  204. (1993). Rational design of potent sialidase-based inhibitors of influenza virus replication.
  205. (2004). Recent trends on the molecular biology of pneumococcal capsules, lytic enzymes, and bacteriophage.
  206. (1994). Receptor specificity of adherence of Streptococcus pneumoniae to human type-II pneumocytes and vascular endothelial cells in vitro.
  207. (2003). Recruitment of murine neutrophils in vivo through endogenous sialidase activity.
  208. (1996). Redox signaling and gene control in the Escherichia coli soxRS oxidative stress regulons: a review.
  209. (1989). Reduced virulence of a defined pneumolysin-negative mutant of Streptococcus pneumoniae.
  210. (2000). Relative roles of pneumolysin and hydrogen peroxide from Streptococcus pneumoniae in inhibition of ependymal ciliary beat frequency.
  211. (1992). Release of reactive oxygen species by guinea pig tracheal epithelial cells in vitro.
  212. (2001). Requirement for capsule in colonization by Streptococcus pneumoniae.
  213. (1997). Role of bacterial Mncofactored superoxide dismutase in oxidative stress responses, nasopharyngeal colonization, and sustained bacteremia caused by Haemophilus influenzae type b.
  214. (2001). Role of genetic resistance in invasive pneumococcal infection: Identification and study of susceptibility and resistance in inbred mouse strains.
  215. (2000). Role of manganese-containing superoxide dismutase in oxidative stress and virulence of Streptococcus pneumoniae.
  216. (2003). Role of neuraminidase in lethal synergism between influenza virus and Streptococcus pneumoniae.
  217. (2000). Role of novel choline binding proteins in virulence of Streptococcus pneumoniae.
  218. (1997). Role of oxidants in microbial pathophysiology.
  219. (1992). Role of Vibrio cholerae neuraminidase in the function of cholera toxin.
  220. (1996). Roles of autolysin and pneumolysin in middle ear inflammation caused by a type 3 Streptococcus pneumoniae strain in the chinchilla otitis media model.
  221. (1999). Safety and immunogenicity of heptavalent pneumococcal CRM197 conjugate vaccine in infants and toddlers. The Pediatric Infectious Disease
  222. (1986). Searching for autolysin functions: characterization of a pneumococcal mutant deleted in the lytA gene.
  223. (2001). Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR.
  224. (1996). Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae.
  225. (1990). Sequence of the Streptococcus pneumoniae bacteriophage HB-3 amidase reveals high homology with the major host autolysin.
  226. (2002). Shortsequence tandem and nontandem DNA repeats and endogenous hydrogen peroxide production contribute to genetic instability of Streptococcus pneumoniae.
  227. (2004). Sialic acid recognition by Vibrio cholerae neuraminidase.
  228. (1997). Sialic acids as ligands in recognition phenomena.
  229. (1990). Sialidase (neuraminidase) activity among Gram-negative anaerobic and capnophilic bacteria.
  230. (1996). Sialidases: Structures, biological significance and therapeutic potential.
  231. (1999). SodA and manganese are essential for resistance to oxidative stress in growing and sporulating cells of Bacillus subtilis.
  232. (2003). Specificity of a third kind: Reactive oxygen and nitrogen intermediates in cell signaling.
  233. (1997). SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component.
  234. (1998). Steroid pulse therapy for rheumatoid arthritis: Effect on lymphocyte subsets and mononuclear cell adhesion.
  235. (1995). Streptococcus pneumoniae anchor to activated human cells by the receptor for platelet-activating factor.
  236. (2004). Streptococcus pneumoniae colonisation: The key to pneumococcal disease.
  237. (2003). Streptococcus pneumoniae damages the ciliated ependyma of the brain during meningitis.
  238. (1999). Streptococcus pneumoniae.
  239. (1995). Stress response in Lactococcus lactis: Cloning, expression analysis, and mutation of the lactococcal superoxide dismutase gene.
  240. (2008). Structural and functional studies of Streptococcus pneumoniae neuraminidase B: An intramolecular transsialidase.
  241. (2006). Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase.
  242. (2008). Structure of the catalytic domain of Streptococcus pneumoniae sialidase NanA.
  243. (1979). Structure of the chromophore of Aequorea green fluorescent protein.
  244. (1953). Studies of the bacterial cell wall. IV. The composition of the cell walls of some gram-positive and gram-negative bacteria.
  245. (1984). Studies on the conformations of sialyloligosaccharides and implications on the binding specificity of neuraminidases.
  246. (1999). Studies on the role of superoxide dismutase (SOD) in the virulence of Streptococcus pneumoniae and the effects of interferon gamma on sensitivity of phagocytes to the toxin pneumolysin.
  247. Subcellular localization of the major pneumococcal autolysin: A peculiar mechanism of secretion in Escherichia coli.
  248. (1999). Superoxide and iron: Partners in crime.
  249. (1997). Superoxide anion radical (O2-.), superoxide dismutases, and related matters.
  250. (2001). Superoxide dismutasedeficient mutants of Helicobacter pylori are hypersensitive to oxidative stress and defective in host colonization.
  251. (1992). Suppression of oxidative envelope damage by pseudoreversion of a superoxide dismutase-deficient mutant of Escherichia coli.
  252. (1999). Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope.
  253. (2009). Surface-associated lipoprotein PpmA of Streptococcus pneumoniae is involved in colonization in a strain-specific manner.
  254. (2006). Switch from planktonic to sessile life: A major event in pneumococcal pathogenesis.
  255. (2008). The ability to utilize mucin affects the regulation of virulence gene expression in Streptococcus pneumoniae.
  256. (1915). The action of ethylhydrocuprein (optochin) on type strains of pneumococci in vitro and in vivo, and on some other microorganisms in vitro
  257. (1998). The adhesion-associated sca operon in Streptococcus gordonii encodes an inducible high-affinity ABC transporter for Mn2+ uptake.
  258. (2001). The autolysin ami contributes to the adhesion of Listeria monocytogenes to eukaryotic cells via its cell wall anchor.
  259. (1998). The crystal structure of pneumococcal surface antigen PsaA reveals a metal-binding site and a novel structure for a putative ABC-type binding protein. Structure (London,
  260. (1981). The development of the bacterial flora in normal neonates.
  261. (2003). The genome sequence of Clostridium tetani, the causative agent of tetanus disease.
  262. (1998). The green fluorescent protein.
  263. (1985). The induction of meningeal inflammation by components of the pneumococcal cell wall.
  264. (2004). The innate immune response to pneumococcal lung infection: The untold story.
  265. (1992). The interaction of Streptococcus pneumoniae with intact human respiratory mucosa in vitro.
  266. (1993). The L-arginine-nitric oxide pathway.
  267. (1999). The molecular characterization of the first autolytic lysozyme of Streptococcus pneumoniae reveals evolutionary mobile domains.
  268. (1989). The molecular genetics of superoxide dismutase in E. coli. An approach to understanding the biological role and regulation of SOD’s in relation to other elements of the defence system against oxygen toxicity.
  269. (2005). The PECACE domain: A new family of enzymes with potential peptidoglycan cleavage activity in Gram-positive bacteria.
  270. (2000). The potential to use PspA and other pneumococcal proteins to elicit protection against pneumococcal infection.
  271. (1995). The production of neuraminidase and fucosidase by Helicobacter pylori: Their possible relationship to pathogenicity.
  272. (1995). The role of pneumolysin and autolysin in the pathology of pneumonia and septicemia in mice infected with a type 2 pneumococcus.
  273. (2006). The role of Streptococcus pneumoniae sortase A in colonisation and pathogenesis.
  274. (1993). The sialidase superfamily and its spread by horizontal gene transfer.
  275. (2006). The uses of green fluorescent protein in prokaryotes in Green fluorescent protein: Properties, applications and protocols.
  276. (1987). Tissue injury in inflammation. Oxidants, proteinases, and cationic proteins.
  277. (2004). Tissuespecific contributions of pneumococcal virulence factors to pathogenesis.
  278. (2003). Todar's online textbook of bacteriology. University of WisconsinMadison Department of Bacteriology. Available from http://www.textbookofbacteriology.net/ Tomasz, A.
  279. (1970). Toxicity of pneumococcal neuraminidase.
  280. (1985). Trace elements in human body fluids and tissues.
  281. (2002). Upper and lower respiratory tract infection by Streptococcus pneumoniae is affected by pneumolysin deficiency and differences in capsule type.
  282. (2001). Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo.
  283. (2004). Vaccination: A major cause of serious illness and mortality, pneumococcal infection is fully preventable by vaccination.
  284. (2006). Variation in the presence of neuraminidase genes among Streptococcus pneumoniae isolates with identical sequence types.
  285. (2006). Versatility of pneumococcal surface proteins.
  286. (2000). Virulence factors and the pathogenesis of disease caused by Streptococcus pneumoniae.
  287. (1993). Wheater's functional histology: A text and colour atlas.
  288. (1993). Wheater’s Functional Histology: a text and colour atlas. 3rd Ed.

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