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Crystal structure of the ascorbate peroxidase-ascorbate complex.

By K.H. Sharp, M. Mewies, Peter C.E. Moody and E.L. Raven

Abstract

Heme peroxidases catalyze the H2O2-dependent oxidation of a variety of substrates, most of which are organic. Mechanistically, these enzymes are well characterized: they share a common catalytic cycle that involves formation of a two-electron, oxidized Compound I intermediate followed by two single-electron reduction steps by substrate. The substrate specificity is more diverse — most peroxidases oxidize small organic substrates, but there are prominent exceptions — and there is a notable absence of structural information for a representative peroxidase−substrate complex. Thus, the features that control substrate specificity remain undefined. We present the structure of the complex of ascorbate peroxidase−ascorbate. The structure defines the ascorbate-binding interaction for the first time and provides new rationalization of the unusual functional features of the related cytochrome c peroxidase enzyme, which has been a benchmark for peroxidase catalysis for more than 20 years. A new mechanism for electron transfer is proposed that challenges existing views of substrate oxidation in other peroxidases

Year: 2003
DOI identifier: 10.1038/nsb913
OAI identifier: oai:lra.le.ac.uk:2381/621
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