Skip to main content
Article thumbnail
Location of Repository

Comparative analysis of the α-like globin clusters in mouse, rat and human chromosomes indicates a mechanism underlying breaks in conserved synteny.

By Cristina Tufarelli, Ross Hardison, Webb Miller, Jim Hughes, Kevin Clark, Nicki Ventress, Anna-Marie Frischauf and Douglas R. Higgs

Abstract

We have sequenced and fully annotated a 65,871-bp region of mouse Chromosome 17 including the Hba-ps4 α-globin pseudogene. Comparative sequence analysis with the functional α-globin loci at human Chromosome 16p13.3 and mouse Chromosome 11 shows that this segment of mouse Chromosome 17 contains a group of three α-like pseudogenes (Hba-psm–Hba-ps4–Hba-q3), similar to the duplicated sets found at the functional mouse cluster on Chromosome 11. In addition, exons 7 to 12 of the mLuc7L gene are present just downstream from the pseudogene cluster, indicating that this clone contains the region in which human 16p13.3 switches in synteny between mouse Chromosomes 11 and 17. Comparison of the sequences around the α-like clusters on the two mouse chromosomes reveals the presence of conserved tandem repeats. We propose that these repetitive elements have played a role in the fragmentation of the mouse α cluster during evolution.Peer-reviewedPublisher Versio

Publisher: Cold Spring Harbor Laboratory
Year: 2004
DOI identifier: 10.1101/gr.2143604
OAI identifier: oai:lra.le.ac.uk:2381/2083
Journal:

Suggested articles

Citations

  1. (1994). 2.2 Mb of contiguous nucleotide sequence from chromosome doi
  2. 4, 2003; accepted in revised form
  3. (1993). A 5 element of the chicken -globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. doi
  4. (1980). A mouse -globin-related pseudogene lacking intervening sequences. doi
  5. (1994). An interstitial telomere array near Hba on mouse Chr 11 is a candidate for the homolog of the telomere at human 16p13.3. doi
  6. (2000). Analysis of the vertebrate SCL loci identifies conserved enhancers. doi
  7. (2001). Characterization of a widely expressed gene (LUC7-LIKE; LUC7L) defining the centromeric boundary of the human -globin domain. doi
  8. (1985). Characterization, expression and evolution of the mouse embryonic -globin gene. doi
  9. (2001). Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the globin cluster. doi
  10. (2000). Comparative maps of human 19p13.3 and mouse chromosome 10 allow identification of sequences at evolutionary breakpoints. doi
  11. (2002). Conserved CTCF insulator elements flank the mouse and human -globin loci. doi
  12. (2000). Conserved noncoding sequences are reliable guides to regulatory elements. doi
  13. (1981). Dispersion of -like globin genes of the mouse to three different chromosomes. doi
  14. (2013). Evolution of the Mouse -Globin Gene Cluster Genome Research 629 www.genome.org
  15. (1993). Genomic rearrangement of the -globin gene complex during mammalian evolution. doi
  16. (1992). Human–mouse homologies in the region of the polycystic kidney disease gene (PKD1). doi
  17. (2001). Identification of a conserved erythroid specific domain of histone acetylation across the -globin gene cluster. doi
  18. (2002). Initial sequencing and comparative analysis of the mouse genome.
  19. (1997). Large-scale comparative sequence analysis of the human and murine Bruton’s tyrosine kinase loci reveals conserved regulatory domains. Genome Res.
  20. (1997). Long range human–mouse sequence alignments reveal novel regulatory elements: A reason to sequence the mouse genome.
  21. (1995). Mapping around the Fused locus on mouse Chromosome 17. doi
  22. (1986). Molecular characterisation of a hypervariable region downstream of the human -globin gene cluster.
  23. (2001). Monosomy for the most telomeric, gene-rich region of the short arm of human chromosome 16 causes minimal phenotypic effects. doi
  24. (1995). Mouse cyclin F maps to a conserved linkage group on mouse chromosome 17. doi
  25. (1980). Mouse globin system: A functional and evolutionary analysis. doi
  26. (2000). PipMaker—A Web server for aligning two genomic DNA sequences. doi
  27. (2001). Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.
  28. (2000). Structural and functional conservation at the boundaries of the chicken -globin domain. doi
  29. (1997). The IL-9 receptor gene, located in the Xq/Yq pseudoautosomal region, has an autosomal origin, escapes X inactivation, and is expressed from the Y. doi
  30. (1996). The mouse homologue of the polycystic kidney disease gene (Pkd1) is a single-copy gene. doi
  31. (1995). The mouse homologue of the tuberin gene (TSC2) maps to a conserved linkage group betwen mouse chromosome 17 and human 16p13.3. doi
  32. (1997). The relationship between chromosome structure and function at a human telomeric region. doi
  33. (1993). Use of long sequence alignments to study the evolution and regulation of mammalian globin gene clusters.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.