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Candida albicans VPS11 is required for vacuole biogenesis and germ tube formation

By Glen E. Palmer, Annette M. Cashmore and J. Sturtevant


The Candida albicans vacuole has previously been observed to undergo rapid expansion during the emergence of a germ tube from a yeast cell, to occupy the majority of the parent yeast cell. Furthermore, the yeast-to-hypha switch has been implicated in the virulence of this organism. The class C vps (vacuolar protein sorting) mutants of Saccharomyces cerevisiae are defective in multiple protein delivery pathways to the vacuole and prevacuole compartment. In this study C. albicans homologues of the S. cerevisiae class C VPS genes have been identified. Deletion of a C. albicans VPS11 homologue resulted in a number of phenotypes that closely resemble those of the class C vps mutants of S. cerevisiae, including the absence of a vacuolar compartment. The C. albicans vps11Δ mutant also had much-reduced secreted lipase and aspartyl protease activities. Furthermore, vps11Δ strains were defective in yeast-hypha morphogenesis. Upon serum induction of filamentous growth, mutants showed delayed emergence of germ tubes, had a reduced apical extension rate compared to those of control strains, and were unable to form mature hyphae. These results suggest that Vps11p-mediated trafficking steps are necessary to support the rapid emergence and extension of the germ tube from the parent yeast cell.Peer-reviewedPublisher Versio

Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/EC.2.3.411-421.2003
OAI identifier:

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  1. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. doi
  2. (2002). A Forkhead transcription factor is important for true hyphal as well as yeast morphogenesis in Candida albicans. doi
  3. (1984). A model for the germ tube formation and mycelial growth form of Candida albicans. doi
  4. (1997). A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. doi
  5. (2000). A phosphatidylinositol 3-kinase of Candida albicans influences adhesion, filamentous growth and virulence. doi
  6. (1991). A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation. doi
  7. (1992). Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction. doi
  8. (1990). Basic local alignment search tool. doi
  9. (1989). Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.
  10. (1989). Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast.
  11. (1990). Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. doi
  12. (2000). Class C Vps protein complex regulates vacuolar SNARE pairing and is required for vesicle docking/fusion. doi
  13. Cloning and characterization of a gene (LIP1) which encodes a lipase from the pathogenic yeast Candida albicans. doi
  14. (1998). Coiled coils in both intracellular vesicle and viral membrane fusion. doi
  15. (1998). Disruption studies of a Candida albicans gene, ELF1, a member of the ATP binding cassette family. doi
  16. (1988). Dynamic aspects of vacuolar and cytosolic amino acid pools of Saccharomyces cerevisiae.
  17. (2001). Functional analysis of the vacuole in Candida albicans.
  18. (1998). Genetic interaction with vps8-200 allows partial suppression of the vestigial vacuole phenotype caused by a pep5 mutation in Saccharomyces cerevisiae.
  19. (1992). Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. doi
  20. (1989). Intracellular targeting and structural conservation of a prohormone-processing endoprotease. doi
  21. (1991). Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae. doi
  22. (1984). Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor. doi
  23. (1986). Isolation of yeast mutants defective in protein targeting to the vacuole. doi
  24. (1997). KEX2 influences Candida albicans proteinase secretion and hyphal formation. doi
  25. (1998). Membrane fusion. SNARE the rod, coil the complex.
  26. (1991). Methods for studying the yeast vacuole. Methods Enzymol. doi
  27. (2000). Methods in yeast genetics:
  28. (2001). Molecular cloning: a laboratory manual, 3rd ed. doi
  29. (1988). Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. doi
  30. (2000). New component of the vacuolar class C-Vps complex couples nucleotide exchange on the Ypt7 GTPase to SNARE-dependent docking and fusion. doi
  31. (1988). Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting. doi
  32. (2000). Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.
  33. (1977). Phagocytosis of Candida albicans by rabbit alveolar macrophages and guinea pig neutrophils. Sabouraudia 15:171–177. doi
  34. (1995). PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis. doi
  35. (1991). Predicting coiled coils from protein sequences. doi
  36. (1981). Protein degradation, meiosis and sporulation in proteinase-deficient mutants of Saccharomyces cerevisiae.
  37. (1988). Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. doi
  38. (1986). Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. doi
  39. (1977). Proteinase mutants of Saccharomyces cerevisiae. doi
  40. (1999). Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.
  41. (2000). RING domains: master builders of molecular scaffolds? doi
  42. (1997). Saccharomyces cerevisiae mutants altered in vacuole function are defective in copper detoxification and iron-responsive gene transcription. doi
  43. (1987). Segregation of proteinase-negative mutants from heterozygous Candida albicans. doi
  44. (1990). The fungal vacuole: composition, function, and biogenesis.
  45. Tracing intracellular proteolytic pathways. Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae.
  46. (2002). Vacuolar proteases and proteolytic artifacts in Saccharomyces cerevisiae. Methods Enzymol. doi
  47. (1982). Vacuolation, branch production and linear growth of germ tubes in Candida albicans. doi
  48. (2001). Virulence factors of Candida albicans. Trends Microbiol. doi
  49. (1993). Vps1p, a member of the dynamin GTPase family, is necessary for Golgi membrane protein retention in Saccharomyces cerevisiae.
  50. (1995). VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. doi

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