Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”

Abstract

Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including liver cancer. In this study, methanolic extracts (MEs) of 23 plants were tested for antiproliferative activity on human hepatoma cell line (Hep G2) by the sulforhodamine B (SRB) assay. Nine MEs with potent antiproliferative activity (IC50&lt; 100 µg/mL) were obtained and further semipurified by liquid/liquid partition extraction. The semipurified fractions were tested for the antiproliferative and antioxidative activities. ME ofStemona collinsaeand the semipurified extract and methanol-water fraction (MF) ofGloriosa superbagave the highest antiproliferative activity on HepG2 which were 4.79- and 50.07-fold cisplatin, respectively. The semipurified fractions showed an increased antiproliferative activity. MF ofCaesalpinia sappanand HF ofSenna alatashowed the highest free radical scavenging and metal chelating activities, respectively. The compound inn-hexane fraction (HF) ofVentilago denticulatawhich showed an increase in antiproliferative activity comparing to its ME was isolated and identified as emodin. This study has demonstrated the potential of the ME fromS. collinsae, MF fromG. superba, and emodin isolated fromV. denticulata, for further development as an antiliver cancer agent.</jats:p