Skip to main content
Article thumbnail
Location of Repository

The relationship between biomarkers of oxidative DNA damage, polycyclic aromatic hydrocarbon DNA adducts, antioxidant status and genetic susceptibility following exposure to environmental air pollution in humans

By Rajinder Singh, Radim J. Sram, Blanka Binkova, Ivan Kalina, Todor A. Popov, Tzveta Georgieva, Seymour Garte, Emanuela Taioli and Peter B. Farmer

Abstract

This is the authors' final draft of the paper published as Mutation Research 2007, 620(1-2), pp.83-92 and includes the revised Scheme 1 which appeared as a corrigendum in Mutation Research 2007, 625(1-2), pp.177-78. The final published version of the original paper is available on Science Direct, doi:10.1016/j.mrfmmm.2007.02.025.Polycyclic aromatic hydrocarbons (PAHs) appear to be significant contributors to the genotoxicity and carcinogenicity of air pollution present in the urban environment for humans. Populations exposed to environmental air pollution show increased levels of PAH DNA adducts and it has been postulated that another contributing cause of carcinogenicity by environmental air pollution may be the production of reactive oxygen species following oxidative stress leading to oxidative DNA damage. The antioxidant status as well as the genetic profile of an individual should in theory govern the amount of protection afforded against the deleterious effects associated with exposure to environmental air pollution. In this study we investigated the formation of total PAH (bulky) and B[a]P DNA adducts following exposure of individuals to environmental air pollution in three metropolitan cities and the effect on endogenously derived oxidative DNA damage. Furthermore the influence of antioxidant status (vitamin levels) and genetic susceptibility of individuals with regard to DNA damage was also investigated. There was no significant correlation for individuals between the levels of vitamin A, vitamin E, vitamin C and folate with M1dG and 8-oxodG adducts as well as M1dG adducts with total PAH (bulky) or B[a]P DNA adducts. The interesting find from this study was the significant negative correlation between the level of 8-oxodG adducts and the level of total PAH (bulky) and B[a]P DNA adducts implying the that the repair of oxidative DNA damage may be enhanced. This correlation was most significant for those individuals that were non smokers or those unexposed to environmental air pollution. Furthermore the significant inverse correlation between 8-oxodG and B[a]P DNA adducts was confined to individuals carrying the wild type genotype for both the GSTM1 and the GSTT1 gene (separately and interacting). This effect was not observed for individuals carrying the null variant

Publisher: Elsevier
Year: 2007
DOI identifier: 10.1016/j.mrfmmm.2007.02.025.
OAI identifier: oai:lra.le.ac.uk:2381/3325

Suggested articles

Citations

  1. (1999). 8-hydroxyguanosine formed in human lung tissues and the association with diesel exhaust particles, Free Radic. doi
  2. (2005). Air pollution and cancer: biomarker studies in human populations, doi
  3. (1997). an environmentally exposed population, doi
  4. (2001). Analysis of 13 32P-DNA postlabeling studies on occupational cohorts exposed to air pollution, doi
  5. (2000). Analysis of aromatic DNA adducts and 7,8-dihydro-8-oxo-2´-deoxyguanosine in lymphocyte DNA from a case-control study of lung cancer involving minority populations, doi
  6. (1996). Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes,
  7. (2004). Biomarkers of exposure to polycyclic aromatic hydrocarbons from environmental air pollution, doi
  8. (2002). Detection of malondialdehyde-DNA adducts in human colorectal mucosa: relationship with diet and the presence of adenomas, Cancer Epidemiol. Biomarkers Prev.
  9. (2003). DNA adduct formation and oxidative stress in colon and liver of Big Blue® rats after dietary exposure to diesel particles,. doi
  10. (1995). DNA adducts and personal air monitoring of carcinogenic polycyclic aromatic hydrocarbons in an environmentally exposed population. doi
  11. (2001). DNA strand breaks, oxidative damage and 1-OH pyrene in roofers with coal-tar pitch dust and/or asphalt fume exposure, doi
  12. (2002). Effect of diet on cancer development: Is oxidative DNA damage a biomarker? Free Radic. doi
  13. Effects of metabolic genotypes on intermediary biomarkers in subjects exposed to PAHs. Results from the EXPAH study, doi
  14. (1999). Environmental tobacco smoke, genetic susceptibility and risk of lung cancer in never-smoking women, doi
  15. (1994). Generation of mutagens during arachidonic acid metabolism, doi
  16. (2004). Genotoxic effects of a complex mixture adsorbed onto ambient air particles on human cells in vitro: the effects of vitamins E doi
  17. (2004). Genotoxicity of airborne particulate matter: he role of cell-particle interaction and of substances with adduct-forming and oxidizing capacity, doi
  18. (2005). Glutathione transferases, doi
  19. (2004). Impact of phase I or phase II enzyme polymorphisms on lymphocyte DNA adducts in subjects exposed to urban air pollution and environmental tobacco smoke, doi
  20. (2004). In vitro and in vivo studies on oxygen free radical and DNA adduct formation in rat lung and liver during benzo[a]pyrene metabolism, Free Radic. doi
  21. (2000). Increase in mutation frequency in lung of Big Blue rat by exposure to diesel exhaust, doi
  22. (1998). Indirect mutagenesis by oxidative DNA damage: Formation of the pyrimidopurinone adduct of deoxyguanosine by base propenal, doi
  23. (2000). Indoor coal combustion emissions, GSTM1 and GSTT1 genotypes and lung cancer risk: A case-control study in Xuan, Cancer Epidemiol. Biomarkers and Prev. doi
  24. (2003). Leukocyte 8-hydroxydeoxyguanosine and aromatic DNA adduct in cokeoven workers with polycylic aromatic hydrocarbon exposure, doi
  25. (2003). Linking exposure to environmental pollutants with biological effects, doi
  26. (1997). Molecular epidemiology of the human glutathione S-transferase genotypes GSTM1 and GSTT1 in cancer susceptibility, Cancer Epidemiol. Biomarkers Prev.
  27. (2003). Oxidative DNA base modifications and polycyclic aromatic hydrocarbon DNA adducts in squamous cell carcinoma of larynx, doi
  28. (2003). Oxidative DNA damage and defence gene expression in the mouse lung after shortterm exposure to diesel exhaust particles by inhalation, doi
  29. (1997). Oxidative stress to DNA, protein and antioxidant enzymes (superoxide dismutase and catalase) in rats treated with benzo[a]pyrene, doi
  30. (2005). Oxidative stress-induced DNA damage by particulate air pollution. doi
  31. (2000). Oxyradicals and DNA damage, doi
  32. (1997). Repair of propanodeoxyguanosine by nucleotide excision repair in vivo and in vitro, doi
  33. (2003). Urinary excretion of 8-hydroxy-2´-deoxyguanosine and 1-hydroxypyrene in coke-oven workers, doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.