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MegaPlex PCR: a strategy for multiplex amplification

By Linda Strömqvist Meuzelaar, Owen Lancaster, J. Paul Pasche, Guido Kopal and Anthony J. Brookes

Abstract

This is the authors' final draft of the paper published as Nature Methods, 2007, 4, pp.835-837. The definitive published version is available from http://www.nature.com/nmeth/, doi:10.1038/nmeth1091‘MegaPlex PCR’ is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by coamplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci

Publisher: Nature Publishing Group
Year: 2007
DOI identifier: 10.1038/nmeth1091
OAI identifier: oai:lra.le.ac.uk:2381/3833

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