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The effects of MSH2 deficiency on spontaneous and radiation-induced mutation rates in the mouse germline

By Karen L.A. Burr, Annemarie van Duyn-Goedhart, Peter Hickenbotham, Paul P.W. van Buul and Yuri E. Dubrova


This is the author's draft of an article published by Elsevier rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of mismatch repair deficient Msh2 knock-out mice. Spontaneous mutation rates in homozygous Msh2-/- males were significantly higher than those in isogenic wild-type (Msh2+/+) and heterozygous (Msh2+/-) mice. In contrast, the irradiated Msh2-/- mice did not show any detectable increases in their mutation rate, whereas significant ESTR mutation induction was observed in the irradiated Msh2+/+ and Msh2+/- animals. Considering these data and the results of other publications, we propose that the Msh2-deficient mice possess a mutator phenotype in their germline and somatic tissues while the loss of a single Msh2 allele does not affect the stability of heterozygotes

Publisher: Elsevier
Year: 2007
OAI identifier:

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