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The effects of MSH2 deficiency on spontaneous and radiation-induced mutation rates in the mouse germline

By Karen L.A. Burr, Annemarie van Duyn-Goedhart, Peter Hickenbotham, Paul P.W. van Buul and Yuri E. Dubrova

Abstract

This is the author's draft of an article published by Elsevier http://www.elsevier.com/wps/find/journaldescription.cws_home/506092/description#descriptionMutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of mismatch repair deficient Msh2 knock-out mice. Spontaneous mutation rates in homozygous Msh2-/- males were significantly higher than those in isogenic wild-type (Msh2+/+) and heterozygous (Msh2+/-) mice. In contrast, the irradiated Msh2-/- mice did not show any detectable increases in their mutation rate, whereas significant ESTR mutation induction was observed in the irradiated Msh2+/+ and Msh2+/- animals. Considering these data and the results of other publications, we propose that the Msh2-deficient mice possess a mutator phenotype in their germline and somatic tissues while the loss of a single Msh2 allele does not affect the stability of heterozygotes

Publisher: Elsevier
Year: 2007
OAI identifier: oai:lra.le.ac.uk:2381/829

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Citations

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