Skip to main content
Article thumbnail
Location of Repository

The efficiency of multilocus DNA fingerprint probes for individualization and establishment of family relationships, determined from extensive casework

By Alec J. Jeffreys, Michelle Turner and Paul Debenham


© 1991 by the American Society of Human Genetics. Also available from PubMed Central at http://www.pubmedcentral.nih.govThe properties of human DNA fingerprints detected by multilocus minisatellite probes 33.6 and 33.15 have been investigated in 36 large sibships and in 1,702 Caucasian paternity cases involving the analysis of over 180,000 DNA fingerprint bands. The degree of overlap of minisatellite loci detected by these two probes is shown to be negligible (~1%), and the resulting DNA fingerprints are therefore derived from independent sets of hypervariable loci. The level of allelism and linkage between different hypervariable DNA fragments\ud scored with these probes is also low, implying substantial statistical independence of DNA fragments. Variation between the DNA fingerprints of different individuals indicates that the probability of chance identity is very low (<<10-7 per probe). Empirical observations and theoretical considerations both indicate\ud that genetic heterogeneity between subpopulations is unlikely to affect substantially the statistical evaluation\ud of DNA fingerprints, at least among Caucasians. In paternity analysis, the proportion of nonmaternal DNA fragments in a child which cannot be attributed to the alleged father is shown to be an efficient statistic for distinguishing fathers from nonfathers, even in the presence of minisatellite mutation. Band-sharing estimates between a claimed parent and a child can also distinguish paternity from nonpaternity, though with less efficiency than comparison of a trio of mother, child, and alleged father

Publisher: University of Chicago Press
Year: 1991
OAI identifier:

Suggested articles


  1. (1988). Application of DNA &quot;fingerprints&quot; to paternity determinations. doi
  2. (1988). Clustering of hypervariable minisatellites in the proterminal regions of human autosomes. doi
  3. (1987). different minisatellite probes. Cytogenet Cell Genet 47:127-Gill
  4. (1991). DNA finger printing by oligonucleotide probes specific for simple repeats. Hum Genet 74:239-243 Armour doi
  5. (1988). Primer-directed enzymatic amplification ofDNA with a thermostable DNA polymerase. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.