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26-locus Y-STR typing in a Bhutanese population sample

By Emma J. Parkin, Thirsa Kraayenbrink, George L. van Driem, Karma Tshering of Gaselo, Peter de Knijff and Mark A. Jobling


This is the author's draft of the version published in Forensic Science International Y-chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. (2002), and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461,\ud DYS462) and also amplifies the amelogenin locus. The 26 loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci, and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype\ud Reference Database

Publisher: Elsevier
Year: 2006
OAI identifier:

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