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Single molecule enzymology: what can it tell us?

By Clive R. Bagshaw

Abstract

Also available via http://www.biochemist.orgIn the last decade, several methods have been developed to measure enzyme activity at the level of a single enzyme molecule. This represents a considerable technical feat, but what does it allow us to learn about enzymes? Here, the basic principles are reviewed to show that new forms of heterogeneity in activity may be revealed and evidence gained for rare states that would otherwise be swamped in bulk assays

Publisher: The Biochemical Society
Year: 2003
OAI identifier: oai:lra.le.ac.uk:2381/147

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Citations

  1. (2001). Single Molecule Spectroscopy, doi

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