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Small-diameter white myotomal muscle fibres associated with growth hyperplasia in the carp (Cyprinus carpio) express a distinct myosin heavy chain gene

By Steven J. Ennion, Laurent Gauvry, Peter Butterworth and Geoffrey Goldspink

Abstract

A carp myosin heavy chain gene isoform was isolated from a genomic clone, restriction mapped and partially sequenced to reveal the location of various exons. The clone\ud contains a complete gene of approximately 12.0 kb which is half the size of the corresponding mammalian and avian myosin heavy chain genes. The mRNA transcript of this gene, however, is the same size as mammalian and avian striated muscle myosin heavy chain genes (about 6000 nucleotides), illustrating that the difference in size at the genomic level is due to shorter introns. A 169 bp NsiI restriction fragment containing only the 39 untranslated\ud region of this gene was subcloned and used as an isoform-specific probe to study the expression of this particular isoform. Hybridisation analysis could only detect\ud expression of this myosin heavy chain gene in the white muscle of adult carp that had been subjected to an increased environmental temperature. No expression of\ud this gene was detected in carp under 1 year of age. In situ hybridisation demonstrated that expression of this gene is\ud limited to small-diameter white muscle fibres of adult carp, which are thought to be responsible for muscle growth by\ud fibre hyperplasia

Publisher: Company of Biologists
Year: 1995
OAI identifier: oai:lra.le.ac.uk:2381/116

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  1. (1983). A comparative study of fibre type characteristics and terminal innervation in head and axial muscleof the carp (Cyprinus carpio L.): a histochemical and electronmicroscopial study. doi
  2. (1975). Adaptations in Mg++-activated myofibrillar ATPase induced by temperature acclimation. doi
  3. (1984). Addenum: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. doi
  4. (1990). Cellular and molecular diversities of mammalian skeletal muscle fibers. doi
  5. (1995). Characterisation of human skeletal muscle fibres according to the myosin heavy chains they express. doi
  6. (1985). Comparative study of myosins present in the lateral muscle of some fish: species variations in myosin isoformsand their distribution in red, pink and white muscle. doi
  7. (1986). Complete nucleotide and encoded amino acid sequence of a mammalian myosin heavy chain gene. Evidence against intron-dependent evolution of the rod. doi
  8. (1991). Complete sequence of human cardiac alpha-myosin heavy chain gene and amino acid comparison to other myosins based on structural and functional differences. doi
  9. (1991). Coordination of skeletal muscle gene expression occurs late in mammalian development. doi
  10. (1988). Developmental transitions in myosin isoforms and organisation of the lateral muscle in the teleost Dicentrarchus labrax (L.). doi
  11. (1995). Differentiation and growth of muscle in the fish Sparus aurata (L.). II. Hyperplastic and hypertrophic growth of lateral muscle from hatching to adult. J. Muscle Res. Cell Motil. doi
  12. (1989). Distribution of fast myosin heavy chain isoforms in thick filaments of developing chicken pectoral muscle. doi
  13. (1990). Disuse and passive stretch cause rapid alterations in expression of developmental and adult contractile protein genes in skeletal muscle.
  14. (1982). Fetal myosin heavy chains in regenerating muscle. doi
  15. (1993). Functional morphology of toadfish sonic muscle fibers – relationship to possible fiber division Can. doi
  16. (1990). Fusion between myoblasts and adult muscle fibers promotes remodelling of fibers into myotubes in vitro.
  17. (1992). Gene conversions within the skeletal myosin multigene family. doi
  18. (1984). Genomic sequencing. doi
  19. (1983). Growth and development of muscle fibres in the rainbow trout (Salmo gairdneri).
  20. (1983). Growth and development of skeletal muscle fibres in the cod. doi
  21. (1984). Growth dynamics of white myotomal muscle fibres in the bluntnose minnow (Pimephales notatus Rafinesque) and comparison with rainbow trout (Salmo gairdneri Richardson). doi
  22. (1993). Growth of carp (Cyprinus carpio) white axial muscle: hyperplasia and hypertrophy in relation to the myonucleus/sarcoplasm ratio and the occurrence of different subclasses of myogenic cells. doi
  23. (1989). Human embryonic myosin heavy chain cDNA. Interspecies sequence conservation of the myosin rod, chromosomal locus and isoform specific transcription of the gene. doi
  24. (1992). In situ hybridisation and immunohistochemistry.
  25. (1993). Influence of fish size on proliferation of cultured myosatellite cells of white axial muscle of carp (Cyprinus carpio L.). doi
  26. (1990). Mechanisms of temperature acclimation in the carp: a molecular biology approach.
  27. (1985). Muscle protein synthesis rates during temperature acclimation in a eurythermal (Cyprinus carpio) and a stenothermal (Salmo gairdneri) species of teleost.
  28. (1987). Polymorphism of contractile proteins. doi
  29. (1972). Postembryonic growth and differentiation of striated skeletal muscle. doi
  30. (1982). Sarcomeric myosin heavy chain is coded by a highly conserved multigene family. doi
  31. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. doi
  32. (1986). The chicken myosin heavy chain family. doi
  33. (1990). The complete sequence of the human beta-myosin heavy chain gene and a comparative analysis of its product. doi
  34. (1987). The sequence of an embryonic myosin heavy chain gene and isolation of its corresponding cDNA.
  35. (1993). Type-2x-myosin heavy chain is coded by a muscle fiber type-specific and developmentally regulated gene. doi

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