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Analysis of transposon insertion mutants highlights the diversity of mechanisms underlying male progamic development in Arabidopsis

By Eric Lalanne, Christos Michaelidis, James M. Moore, Wendy Gagliano, Andrew Johnson, Ramesh Patel, Ross Howden, Jean-Phillippe Vielle-Calzada, Ueli Grossniklaus and David Twell

Abstract

To identify genes with essential roles in male gametophytic development, including postpollination (progamic) events, we have undertaken a genetic screen based on segregation ratio distortion of a transposon-borne kanamycin-resistance marker. In a population of 3359 Arabidopsis Ds transposon insertion lines, we identified 20 mutants with stably reduced segregation ratios arising from reduced gametophytic transmission. All 20 mutants showed strict cosegregation of Ds and the reduced gametophytic transmission phenotype. Among these, 10 mutants affected both male and female transmission and 10 mutants showed male-specific transmission defects. Four male and female (ungud) mutants and 1 malespecific mutant showed cellular defects in microspores and/or in developing pollen. The 6 remaining ungud mutants and 9 male-specific (seth) mutants affected pollen functions during progamic development. In vitro and in vivo analyses are reported for 5 seth mutants. seth6 completely blocked pollen germination,\ud while seth7 strongly reduced pollen germination efficiency and tube growth. In contrast, seth8, seth9, or seth10 pollen showed reduced competitive ability that was linked to slower rates of pollen tube growth. Gene sequences disrupted in seth insertions suggest essential functions for putative SETH proteins in diverse processes including protein anchoring, cell wall biosynthesis, signaling, and metabolism

Publisher: Genetics Society of America
Year: 2004
OAI identifier: oai:lra.le.ac.uk:2381/89

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  1. (2001). a 150 kb chromosomal deletion adjacent to an introduced
  2. (2003). A calmodulin-binding doi
  3. A high-throughput Arabidopsis reverse genetics system. doi
  4. (2003). A tumor suppressor homolog, AtPTEN1, is essential for halfman, an Arabidopsis male gametophytic mutant associated withpollen development in Arabidopsis.
  5. (2002). Afamily get-together.
  6. (1998). Asymmetric divisionArabidopsis thaliana.
  7. (1985). Comparative analysis of the Arabi-cerning the cellular functions that operate during male dopsis pollen transcriptome.
  8. (2002). families indicates each has essential functions.
  9. (2003). Female control of male gamete delivery during fertilization in UK. doi
  10. (1995). fication of gametophytic mutations affecting female gametophyte
  11. (1999). Five gametophytic mutations affecting pollen devel- cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation.opment and pollen tube growth in Arabidopsis thaliana. Genetics 158: 1773–1783.
  12. (1998). Further systematic screens of
  13. (2002). Genetic control of male germ unit doi
  14. (2002). Gibberellinstube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels. Cell 114: 47–59. are required for seed development and pollen tube growth in Arabidopsis. doi
  15. (1994). Grossniklaus,Cloning and characterization of a maize pollen-specific calcium1997 Genetic characterization of hadad, a mutant disruptingdependent calmodulin-independent protein kinase. doi
  16. (1999). Isolation and characterization of cDNAGagliano,
  17. (1995). Jones etgametophytic mutation gemini pollen disrupts microspore polarity, division asymmetry and pollen cell fate. Development 125: al.,
  18. (2003). phytic genes in Arabidopsis is unexpectedly rare and cannot
  19. (2003). Pilot etAtCSLA7, a cellulose synthase-like putative glycosyltransferase, is al.,
  20. (2000). Plant fructokinases: a sweet Dev. doi
  21. (2002). Pollen developmental biology, doi
  22. (2003). Pollen tube guidance. doi
  23. (1999). Rac homologues and compartmentalized phosphatidylinositol 4,LITERATURE CITED 5-bisphosphate act in a common pathway to regulate polar doi
  24. (2000). RPT2. A signal and cell fate determination in developing pollen. Trends Plant Sci. 3: 305–310.transducer of the phototropic response in Arabidopsis. doi
  25. (1996). Self-sterility in Arabidopsis due doi
  26. (1990). Sequence et al.,
  27. (1996). sidecar pollen, an Arabidopsis
  28. (1994). Tetrad analysis possible cysteine-rich extracellular protein, LAT52, interacts with the extracellular domain of the pollen receptor kinase LePRK2. Plantin Arabidopsis with mutation of the QUARTET (QRT) genes.
  29. (2002). The art and design of doi
  30. (2003). was supported by the Cold Spring Harbor doi

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