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SAFlex: A structural alphabet extension to integrate protein structural flexibility and missing data information

By Ikram Allam, Delphine Flatters, Géraldine Caumes, Leslie Regad, Vincent Delos, Grégory Nuel and Anne-Claude Camproux


International audienceIn this paper, we describe SAFlex (Structural Alphabet Flexibility), an extension of an existing structural alphabet (HMM-SA), to better explore increasing protein three dimensional structure information by encoding conformations of proteins in case of missing residues or uncertainties. An SA aims to reduce three dimensional conformations of proteins as well as their analysis and comparison complexity by simplifying any conformation in a series of structural letters. Our methodology presents several novelties. Firstly, it can account for the encoding uncertainty by providing a wide range of encoding options: the maximum a posteri-ori, the marginal posterior distribution, and the effective number of letters at each given position. Secondly, our new algorithm deals with the missing data in the protein structure files (concerning more than 75% of the proteins from the Protein Data Bank) in a rigorous proba-bilistic framework. Thirdly, SAFlex is able to encode and to build a consensus encoding from different replicates of a single protein such as several homomer chains. This allows localizing structural differences between different chains and detecting structural variability, which is essential for protein flexibility identification. These improvements are illustrated on different proteins, such as the crystal structure of an eukaryotic small heat shock protein. They are promising to explore increasing protein redundancy data and obtain useful quantification of their flexibility

Topics: [MATH.MATH-PR]Mathematics [math]/Probability [math.PR], [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], [INFO.INFO-MS]Computer Science [cs]/Mathematical Software [cs.MS]
Publisher: 'Public Library of Science (PLoS)'
Year: 2018
OAI identifier: oai:HAL:hal-02043567v1
Provided by: HAL Descartes
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