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Foliar exposure of the crop Lactuca sativa to silver nanoparticles: Evidence for internalization and changes in Ag speciation

By Camille Larue, Hiram Castillo-Michel, Sophie Sobanska, Lauric Cécillon, Sarah Bureau, Véronique Barthes, Laurent Ouerdane, Marie Carrière and Géraldine Sarret

Abstract

International audienceThe impact of engineered nanomaterials on plants, which act as a major point of entry of contaminants into trophic chains, is little documented. The foliar pathway is even less known than the soil-root pathway. However, significant inputs of nanoparticles (NPs) on plant foliage may be expected due to deposition of atmospheric particles or application of NP-containing pesticides. The uptake of Ag-NPs in the crop species Lactuca sativa after foliar exposure and their possible biotransformation and phytotoxic effects were studied. In addition to chemical analyses and ecotoxicological tests, micro X-ray fluorescence, micro X-ray absorption spectroscopy, time of flight secondary ion mass spectrometry and electron microscopy were used to localize and determine the speciation of Ag at sub-micrometer resolution. Although no sign of phytotoxicity was observed, Ag was effectively trapped on lettuce leaves and a thorough washing did not decrease Ag content significantly. We provide first evidence for the entrapment of Ag-NPs by the cuticle and penetration in the leaf tissue through stomata, for the diffusion of Ag in leaf tissues, and oxidation of Ag-NPs and complexation of Ag(+) by thiol-containing molecules. Such type of information is crucial for better assessing the risk associated to Ag-NP containing products

Topics: Ecotoxicology, Plants, ToF-SIMS, micro-XAS, Ag nanoparticles, Biodistribution, [SDV]Life Sciences [q-bio], [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Publisher: 'Elsevier BV'
Year: 2014
DOI identifier: 10.1016/j.jhazmat.2013.10.053
OAI identifier: oai:HAL:hal-01974700v1
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