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A comparative study of cationic formulations for the delivery of siRNA and DNA

By N.P.A. Kwok

Abstract

RNA interference (RNAi) provides a specific and efficient way to silence gene expression; therefore, it is an attractive tool to be used in basic research on gene function as well as gene therapy. Despite the enormous potential of RNAi, delivering the small interfering RNA (siRNA) to the cells is one of the main hurdles. Previously there have been reports showing effective plasmid DNA delivery to cells and such systems could be used to deliver siRNA to cells due to the similarity of the delivery criteria between plasmid DNA and siRNA. Therefore, I hypothesise that a successful siRNA delivery system will have similar biophysical characteristics as a successful DNA delivery system. The aim of this study is to identify the important criteria to establish a promising siRNA delivery system by comparing the criteria of successful DNA delivery systems such as linear and branched polylysines and linear and branched PEIs. In order to deliver nucleic acid to a cell, a vector system should be able to bind and form a positively surface-charged nano-sized complex with the nucleic acid for cellular binding and uptake. Inside the cell, the vector should be able to dissociate from the nucleic acid for gene expression or silencing. Therefore, the important parameters to investigate are the binding and dissociation properties of the vector components to the nucleic acid and the size and surface charge of the complex. From the results, generally all the polylysines and PEIs can bind, dissociate and form a positively charged nano-particle with plasmid DNA, which can mediate gene expression. Despite the ability of all the polylysines and PEIs to bind to and dissociate from siRNA, only branched polylysines, linear and branched PEI, but not linear polylysines can form positively charged nano-particles with siRNA. Indeed, branched PEI behaves similarly towards siRNA and DNA biophysically. Interestingly, only branched PEI and siRNA complexes can mediate cellular uptake and 60% target gene knockdown. Branched polylysines or linear PEI siRNA complexes cannot mediate gene silencing in spite of the formation of positively charged nano-particles. This could be due to poor cellular uptake of these complexes or degradation of siRNA upon uptake. Therefore, to improve the design of the siRNA vector system, there is a need to research the cellular binding and uptake of siRNA complexes in the future

Publisher: UCL (University College London)
Year: 2009
OAI identifier: oai:eprints.ucl.ac.uk.OAI2:17424
Provided by: UCL Discovery

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Citations

  1. (2006). 3' UTR seed matches, but not overall identity, are associated with RNAi off-targets",
  2. (2000). A chromatin insulator protects retrovirus vectors from chromosomal position effects",
  3. (2009). A Dose Escalation Trial of an Intravitreal Injection of Sirna027 in Patients With Subfoveal Choroidal Neovascularization (CNV) Secondary to AgeRelated Macular Degeneration (AMD)”, ClinicalTrial.gov web site [online]
  4. (2002). A microRNA in a multiple-turnover RNAi enzyme complex",
  5. (2004). A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin",
  6. (1991). A novel cationic liposome reagent for efficient transfection of mammalian cells",
  7. (1987). A novel method for transfection and expression of reconstituted DNA-protein complexes in eukaryotic cells",
  8. (2002). A tragic setback",
  9. (1997). A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus",
  10. (2005). A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids",
  11. (1995). A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine",
  12. (2007). AAV vector integration sites in mouse hepatocellular carcinoma",
  13. (2003). Adenovirus endocytosis",
  14. (2004). Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo",
  15. (2008). Alnylam Achieves First Human Proof of Concept for an RNAi Therapeutic with GEMINI study”, Alnylam Pharmaceuticals web site [online]
  16. (1998). An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery",
  17. (1997). An Update on Non-clathrin-coated Endocytosis",
  18. (2004). Antisense strategies",
  19. (2003). Antisense technologies. Improvement through novel chemical 306 modifications",
  20. (2003). Approaches for the sequence-specific knockdown of mRNA",
  21. (2005). Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies",
  22. (2005). Argonaute2 cleaves the anti-guide 311 strand of siRNA during RISC activation",
  23. (2008). Artificial envelopment of nonenveloped viruses: enhancing adenovirus tumor targeting in vivo",
  24. (2008). Artificial miRNAs mitigate shRNA-mediated toxicity in the brain: implications for the therapeutic development of RNAi",
  25. (2004). Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo",
  26. (2009). Benitec and City of Hope human trial update", Benitec Limited web site [online] and http://www.abnnewswire.net/media/en/docs/60084-ASX-BLT-538981.pdf>
  27. (1997). Biochemical and functional characterization of DNA complexes capable of targeting genes to hepatocytes via the asialoglycoprotein receptor",
  28. (2001). Biological basket weaving: formation and function of clathrin-coated vesicles",
  29. (2006). Biophysical and structural characterization of polyethylenimine-mediated siRNA delivery in vitro",
  30. (2007). Biophysical characterization of an integrin-targeted lipopolyplex gene delivery vector",
  31. (2002). Breakthrough of the year. Small RNAs make big splash",
  32. (2004). Bringing the role of mRNA decay in the control of gene expression into focus",
  33. (2006). Casas-Mollano
  34. (2005). Cationic lipids enhance siRNA-mediated interferon response in mice",
  35. (2003). Cationic liposome-mediated delivery of siRNAs in adult mice",
  36. (1989). Cationic liposome-mediated transfection",
  37. (2002). Caveolae and caveolin in immune cells: distribution and functions",
  38. (2003). Caveolae-mediated internalization of extracellular HIV-1 tat fusion proteins visualized in real time",
  39. (2001). Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER",
  40. (1999). Caveolins, liquid-ordered domains, and signal transduction",
  41. (2003). Caveosomes and endocytosis of lipid rafts",
  42. (1994). Cell binding and internalization by filamentous phage displaying a cyclic Arg-Gly-Asp-containing peptide",
  43. (2000). Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus",
  44. (2000). Cell delivery, intracellular trafficking and expression of an integrin-mediated gene transfer 298 vector in tracheal epithelial cells",
  45. (2003). Cell surface adherence and endocytosis of protein transduction domains",
  46. (2003). Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake",
  47. (2004). Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement",
  48. (2004). Chances and pitfalls of cell penetrating peptides for cellular drug delivery",
  49. (2000). Characterization of commercially available and synthesized polyethylenimines for gene delivery",
  50. (2007). Characterization of gene delivery in vitro and in vivo by the arginine peptide system",
  51. (2001). Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo",
  52. (2005). Choice of the adequate detection time for the accurate evaluation of the efficiency of siRNA-induced gene silencing",
  53. (1997). Clathrin-coated vesicle formation and protein sorting: an integrated process",
  54. (2008). Clathrin-independent internalization and recycling",
  55. (2001). Clathrin-mediated endocytosis: membrane factors pull the trigger",
  56. (1997). Cleavage of single strand RNA adjacent to RNADNA duplex regions by Escherichia coli RNase H1",
  57. (2009). Comparative study of anti-hepatitis B virus RNA interference by double-stranded adenoassociated virus serotypes 7, 8, and 9",
  58. (2003). Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2'-O-methyl RNA, phosphorothioates and small interfering RNA",
  59. (2006). Control of translation and mRNA degradation by miRNAs and siRNAs",
  60. (2008). Controlled delivery of plasmid DNA and siRNA to intracellular targets using ketalized polyethylenimine",
  61. (2006). Current status of polymeric gene delivery systems",
  62. (2006). Delivery of RNA interference",
  63. (2006). Development and characterisation of chitosan nanoparticles for siRNA delivery",
  64. (2005). Different delivery methods-different expression profiles",
  65. (2005). Disruption of GW bodies impairs mammalian RNA interference",
  66. (1999). DNA complexing with polyamidoamine dendrimers: implications for transfection",
  67. (2009). Dynamic Light Scattering", Malvern Instruments Ltd. web site [online] _scattering.htm> Malvern Instruments Ltd. 2009b, "Zeta potential measurement using laser Doppler electrophoresis", Malvern Instruments Ltd. web site [online]
  68. (2007). Effective RNAi-mediated gene silencing without interruption of the endogenous microRNA pathway",
  69. (2007). Effectiveness of siRNA uptake in target tissues by various delivery methods",
  70. (2003). Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A comparative analysis",
  71. (2002). Emerging clinical applications of RNA",
  72. (2004). Endocytic recycling",
  73. (2000). Enhanced gene expression in mouse lung after PEI-DNA aerosol delivery",
  74. (1999). Enhancement of dendrimer-mediated transfection using synthetic lung surfactant exosurf neonatal in vitro",
  75. (2001). Epstein-Barr virus/human vector provides high-level, long-term expression of alpha1-antitrypsin in mice",
  76. (1995). Evaluation in vitro and in vivo of cationic liposome-expression construct complexes for cystic fibrosis gene therapy",
  77. (2002). Evaluation of lipid-based reagents to mediate intracellular gene delivery",
  78. (2000). Examination of the biophysical interaction between plasmid DNA and the polycations, polylysine and polyornithine, as a basis for their differential gene transfection in-vitro",
  79. (1983). Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate",
  80. (2008). Expression of shRNA from a tissue-specific pol II promoter is an effective and safe RNAi therapeutic",
  81. (2003). Expression profiling reveals off-target gene regulation by RNAi",
  82. (1999). Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery",
  83. (2006). Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways",
  84. (1990). Firefly luciferase : A new tool for molecular biologists,
  85. (2008). Fluorescent proteins as biomarkers and biosensors: throwing color lights on 313 molecular and cellular processes",
  86. (1996). Folate-targeted, anionic liposome-entrapped polylysinecondensed DNA for tumor cell-specific gene transfer",
  87. (2003). Formation of LID vector complexes in water alters physicochemical properties and enhances pulmonary gene expression in vivo",
  88. (2005). Fully 2'-modified oligonucleotide duplexes with improved in vitro potency and stability compared to unmodified small interfering RNA",
  89. (1995). Gene delivery and expression mediated by an integrin-binding peptide",
  90. (1997). Gene therapy -- promises, problems and prospects",
  91. (2004). Gene therapy for cystic fibrosis: an example for lung gene therapy",
  92. (2004). Gene therapy in clinical medicine",
  93. (2004). Gene therapy progress and prospects: electroporation and other physical methods",
  94. (2006). Gene therapy progress and prospects: non-viral gene therapy by systemic delivery",
  95. (2004). Gene therapy progress and prospects: recombinant adeno-associated virus (rAAV) vectors",
  96. (2007). Gene therapy progress and prospects: ultrasound for gene transfer",
  97. (2008). Gene Therapy Trials Worldwide, The Journal of Gene Medicine, John Wiley and Sons Inc. Available from: El Aneed,
  98. (2001). Gene therapy. Safer and virus-free?",
  99. (2004). Gene therapy. Side effects sideline hemophilia trial",
  100. (1997). Gene therapy",
  101. (1990). Gene transfer into humans--immunotherapy of patients with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction", N.Engl.J.Med.,
  102. (2003). Gene transfer to subdermal tissues via a new gene gun design",
  103. (2003). Harmful potential of viral vectors fuels doubts over gene therapy",
  104. (2005). Human RISC couples microRNA biogenesis and posttranscriptional gene silencing",
  105. (2005). Human safety and genetically modified plants: a review of antibiotic resistance markers and future transformation selection technologies",
  106. (2002). Hurdles and Hopes for Cancer Treatment"
  107. (2004). Hydrodynamics-based procedure involves transient hyperpermeability in the hepatic cellular membrane: implication of a nonspecific process in efficient intracellular gene delivery",
  108. (2003). Hygromycin B-selected cell lines from GAL4-regulated pUAST constructs",
  109. (2000). Improving gene therapy's tool kit",
  110. (2007). In vivo gene silencing in solid tumors by targeted electrically mediated siRNA delivery",
  111. (1989). Increased expression of DNA cointroduced with nuclear protein in adult rat liver",
  112. (1992). Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle",
  113. (2003). Inhibition of hepatitis B virus replication in vivo by nucleoside analogues and siRNA",
  114. (2004). Inhibition of influenza virus production in virus-infected mice by RNA interference",
  115. (2005). Inhibition of respiratory viruses by nasally administered siRNA",
  116. (2006). Innovative nanotechnologies for the delivery of oligonucleotides and siRNA",
  117. (2006). Insights into the kinetics of siRNA-mediated gene silencing from live-cell and live-animal bioluminescent imaging",
  118. (2007). Interplay of polyethyleneimine molecular weight and oligonucleotide backbone chemistry in the dynamics of antisense activity",
  119. (2005). Intracellular delivery of large molecules and small particles by cell-penetrating proteins and peptides",
  120. (1990). Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans",
  121. (2007). Intronic microRNA precursors that bypass Drosha processing”,
  122. (2005). Involvement of microRNA in AU-rich element-mediated mRNA instability",
  123. (2004). Killing the messenger",
  124. (2004). Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cells",
  125. (2009). Knocking down barriers: advances in siRNA delivery",
  126. (2006). Linear topology confers in vivo gene transfer activity to polyethylenimines",
  127. (2005). Lipid carriers for gene therapy",
  128. (1998). Lipid-mediated enhancement of transfection by a nonviral integrin-targeting vector",
  129. (1987). Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure",
  130. (2009). Localization of double-stranded small interfering RNA to cytoplasmic processing bodies is
  131. (2005). Long-term inhibition of HIV-1 infection in primary hematopoietic cells by lentiviral vector delivery of a triple combination of anti-HIV shRNA, anti-CCR5 ribozyme, and a nucleolar-localizing TAR decoy."
  132. (2003). Looking into the safety of AAV vectors",
  133. (1997). Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells",
  134. (1995). Macropinocytosis",
  135. (2009). Many roads to maturity: microRNA biogenesis pathways and their regulation”,
  136. (2005). Mechanics of receptor-mediated endocytosis",
  137. (2004). Mechanism of improved gene transfer by the N-terminal stearylation of octaarginine: enhanced cellular association by hydrophobic core formation",
  138. (2008). Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides",
  139. (1999). Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides",
  140. (1995). Mechanisms of nuclear protein import",
  141. (1996). Mechanisms of phagocytosis",
  142. (2003). Medicine. Gene therapy--new challenges ahead",
  143. (2001). Melittin enables efficient vesicular escape and enhanced nuclear access of nonviral gene delivery vectors",
  144. (2006). MicroRNA biogenesis: isolation and characterization of the microprocessor complex",
  145. (2008). Misinterpreting the therapeutic effects of siRNA caused by immune stimulation",
  146. (2008). Modification of adenovirus gene transfer vectors with synthetic polymers: a scientific review and technical guide",
  147. (2003). Molecular basis for the immunostimulatory activity of guanine nucleoside analogs: activation of Toll-like receptor 7",
  148. (2001). Molecular cloing: A laboratory manual,
  149. (1997). Nanoscopic structure of DNA condensed for gene delivery",
  150. (2000). Nonviral gene therapy: promises and challenges.
  151. (2005). Nuclear delivery of macromolecules: barriers and carriers",
  152. (2008). Octaarginineand octalysine-modified nanoparticles have different modes of endosomal escape",
  153. (2005). Oligonucleotide structure influences the interactions between cationic polymers and oligonucleotides",
  154. (2007). OPKO Health Initiates Phase 3 Trial of Bevasiranib for the Treatment of AMD”, Opko Health web site [online] 310 Opko Health.,
  155. (1996). Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold",
  156. (2007). P-body formation 299 is a consequence, not the cause, of RNA-mediated gene silencing",
  157. (1995). par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed",
  158. (2003). PCR primer,
  159. (1995). Persistence length of RNA",
  160. (1987). pH-sensitive immunoliposomes mediate target-cellspecific delivery and controlled expression of a foreign gene in mouse",
  161. (1999). Poly(ethylenimine) and its role in gene delivery",
  162. (1999). Polycation-based DNA complexes for tumor-targeted gene delivery in vivo",
  163. (2001). Polyethylenimine-mediated gene delivery: a mechanistic study",
  164. (2002). Polylysine and polyornithine gene transfer complexes: a study of complex stability and cellular uptake as a basis for their differential in-vitro transfection efficiency",
  165. (2007). Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery",
  166. (2006). Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing",
  167. (2001). Post-transcriptional gene silencing by double-stranded RNA",
  168. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans",
  169. (2003). Practical Flow Cytometry, 4 th edition,
  170. (1998). Preparation of chitosan self-aggregates as a gene delivery system",
  171. (2003). Progress and problems with the use of viral vectors for gene therapy",
  172. (1996). Putative role of chloroquine in gene transfer into a human hepatoma cell line by DNA/lactosylated polylysine complexes",
  173. (1992). Quelling: transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences",
  174. (2004). Rational siRNA design for RNA interference",
  175. (2006). Recent developments in the application of plasmid DNA-based vectors and small interfering RNA therapeutics for cancer",
  176. (1985). Receptor-mediated endocytosis: concepts emerging from the LDL receptor system",
  177. (2005). Regulatable gene expression systems for gene therapy applications: progress and future challenges",
  178. (1994). Regulated internalization of caveolae",
  179. (2003). Regulated portals of entry into the cell",
  180. (1996). Regulation of protein transport to the nucleus: central role of phosphorylation",
  181. (2008). Relating chemical and biological diversity space: a tunable system for efficient gene transfection",
  182. (2007). Repression of protein synthesis by miRNAs: how many mechanisms?",
  183. (2008). Retargeting polymercoated adenovirus to the FGF receptor allows productive infection and mediates efficacy in a peritoneal model of human ovarian cancer",
  184. (2006). RNA interference in vitro and in vivo using a novel chitosan/siRNA nanoparticle 303 system",
  185. (2004). RNA interference using boranophosphate siRNAs: structure-activity relationships",
  186. (2005). RNA interference: from gene silencing to genespecific therapeutics",
  187. (2005). RNAimediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo",
  188. (2001). Role for a bidentate ribonuclease in the initiation step of RNA interference",
  189. (2008). Safety and efficacy of gene transfer for Leber's congenital amaurosis", N.Engl.J.Med.,
  190. (2008). Self-inactivating gammaretroviral vectors for gene therapy of X-linked severe combined immunodeficiency",
  191. (1982). Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena",
  192. (2005). Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA",
  193. (2005). Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7",
  194. (2006). Short interfering RNA strand selection is independent of dsRNA processing polarity during RNAi in Drosophila",
  195. (2009). Short non-coding RNA biology and neurodegenerative disorders: novel disease targets and therapeutics',
  196. (1998). Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system",
  197. (2003). Small interfering RNA inhibits hepatitis B virus replication in mice",
  198. (2007). Sonoporation mediated transduction of pDNA/siRNA into joint synovium in vivo",
  199. (1999). Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver",
  200. (2007). Strategies for silencing human disease using RNA interference",
  201. (2005). Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein",
  202. (2002). Structure and function correlation in histone H2A peptide-mediated gene transfer",
  203. (2000). Structure and function of lipid-DNA complexes for gene delivery",
  204. (2003). Structure-activity relationship in cationic lipid mediated gene transfection",
  205. (2006). Successful 300 reconstitution of immunity in ADA-SCID by stem cell gene therapy following cessation of PEG-ADA and use of mild preconditioning",
  206. (2008). Surfacemodified and internally cationic polyamidoamine dendrimers for efficient siRNA delivery",
  207. (2009). Sustained long-term RNA interference in nucleus pulposus cells in vivo mediated by unmodified small interfering RNA",
  208. (2008). Systemic delivery of HK Raf-1 siRNA polyplexes inhibits MDA-MB-435 xenografts",
  209. (2007). Systemic siRNA delivery via hydrodynamic intravascular injection",
  210. (1995). T lymphocyte-directed gene therapy for ADA- SCID: initial trial results after 4 years",
  211. (2005). Tat peptide-mediated cellular delivery: back to basics",
  212. (2008). Tf-lipoplexes for neuronal siRNA delivery: a promising system to mediate gene silencing in the CNS",
  213. (2005). The contributions of dsRNA structure to Dicer specificity and efficiency",
  214. (2002). The druggable genome",
  215. (2004). The future of gene therapy",
  216. (1998). The green fluorescent protein",
  217. (1997). The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes",
  218. (2004). The ins and outs of RNAi in mammalian cells",
  219. (2007). The mirtron pathway generates microRNA-class regulatory RNAs in
  220. (1983). The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme",
  221. (2001). The role of caveolae and caveolin in vesicle-dependent and vesicle-independent trafficking",
  222. (1995). The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer",
  223. (2008). The role of macromolecular architecture in passively targeted polymeric carriers for drug and gene delivery",
  224. (1998). The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells",
  225. (2003). The Tolllike receptor 7 (TLR7)-specific stimulus loxoribine uncovers a strong relationship within the TLR7, 8 and 9 subfamily",
  226. (2005). Therapeutic EphA2 gene targeting in vivo using neutral liposomal small interfering RNA delivery",
  227. (2003). Therapeutic potential of antisense oligonucleotides as modulators of alternative splicing",
  228. (2004). Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs",
  229. (1995). Transfection of folate-polylysine DNA complexes: evidence for lysosomal delivery",
  230. (2001). Transfer of full-length Dmd to the diaphragm muscle of Dmd(mdx/mdx) mice through systemic administration of plasmid DNA",
  231. (2007). Transvascular delivery of small interfering RNA to the central nervous system",
  232. (1998). Ultrastructural characterization of cationic liposome-DNA complexes showing enhanced stability in serum and high transfection activity in vivo",
  233. (2003). Uptake of analogs of penetratin, Tat(48-60) and oligoarginine in live cells",
  234. (2006). Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery",
  235. (2000). Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery",

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