Article thumbnail
Location of Repository

Mycobacterium tuberculosis lineage: a naming of the parts

By T.D. McHugh, S.L. Batt, R.J. Shorten, R.D. Gosling, L. Uiso and S.H. Gillespie


There have been many reports of groups of related Mycobacterium tuberculosis strains described variously as lineages, families or clades. There is no objective definition of these groupings making it impossible to define relationships between those groups with biological advantages. Here we describe two groups of related strains obtained from an epidemiological study in Tanzania which we define as the Kilimanjaro and Meru lineages on the basis of IS6110 restriction fragment length polymorphism (RFLP), polymorphic GC rich sequence (PGRS) RFLP and mycobacterial interspersed repeat unit (MIRU) typing. We investigated the concordance between each of the typing techniques and the dispersal of the typing profiles from a core pattern. The Meru lineage is more dispersed than the Kilimanjaro lineage and we speculate that the Meru lineage is older. We suggest that this approach provides an objective definition that proves robust in this epidemiological study. Such a framework will permit associations between a lineage and clinical or bacterial phenomenon to be tested objectively. This definition will also enable new putative lineages to be objectively tested

Topics: Mycobacterium tuberculosis, molecular epidemiology, lineage, IS6110, MIRU, PGRS, Tanzania
Year: 2005
OAI identifier:
Provided by: UCL Discovery

Suggested articles


  1. (1998). 177: 1107-11.Figure Legend
  2. (1997). A city-wide outbreak of a multiple-drugresistant strain of Mycobacterium tuberculosis in New York. Int J Tuberc Lung Dis.
  3. (2001). Automated high-through-put genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units.
  4. (2001). Comparing genomes within the species Mycobacterium tuberculosis. Genome Res.
  5. Evolutionary relationships amongst strains of Mycobacterium tuberculosis with few copies of IS6110.
  6. (2000). False molecular clusters due to nonrandom association of IS6110 with Mycobacterium tuberculosis.
  7. (1998). Genetic susceptibility to mycobacteria and other infectious pathogens in humans.
  8. (2003). Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics. Infect Genet Evol.
  9. (2002). Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol.
  10. (1999). Interpreting DNA fingerprint clusters of Mycobacterium tuberculosis. European Concerted Action on Molecular Epidemiology and Control of Tuberculosis. Int J Tuberc Lung Dis.
  11. (1995). Molecular epidemiology of tuberculosis in London
  12. (2001). Mycobacterium tuberculosis Beijing genotype strains associated with febrile response to treatment. Emerg Infect Dis.
  13. (1998). Nonrandom association of IS6110 and Mycobacterium tuberculosis: implications for molecular epidemiological studies.
  14. (2002). Predominace of a novel Mycobacterium tuberculosis genotype in the Delhi region of India. Tubercul.
  15. (1995). Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania. Trans R Soc Trop Med Hyg.
  16. (1997). Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology.
  17. (1993). Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: Recommendations for a standardized methodology.
  18. (2001). The evolution of mycobacterial pathogenicity: clues from comparative genomics. Trends Microbiol.
  19. (2002). The fitness of a multiple drug resistant Mycobacterium tuberculosis strain changes after transmission between hosts during and outbreak.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.