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Immune responses to V antigen of Yersinia pestis co-encapsulated with IFN-y;: effect of dose and formulation

By K. F. Griffin, Barbara R Conway, H.O. Alpar and E.D. Williamson


Induction of systemic immune responses after intraperitoneal inoculation of poly(L)lactide microspheres containing the V antigen of Yersinia pestis co-encapsulated with IFN-γ were investigated. Serum antibody responses and T cell proliferative responses were measured in groups of Balb/c mice which were injected intraperitoneally with single or double emulsion preparations of either V/IFN-γ or V alone in a range of dose levels. Groups which received V antigen co-encapsulated with IFN-γ produced higher V-specific antibody responses, predominantly of the IgG1 isotype. Administration of 25 μg V/IFN-γ in a single emulsion resulted in a significantly increased (p<0.05) splenic T cell proliferative response to V antigen compared with other formulations. It was concluded that IFN-γ co-encapsulated with V antigen in poly(L)lactide microspheres acted as an adjuvant and increased antigen specific systemic immune responses. Therefore, co-encapsulation with IFN-γ may result in effective single dose vaccines by increasing the immunogenicity of the formulations

Topics: Q1, QD
Publisher: Elsevier
Year: 1998
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