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The adenylate cyclase activity in heart membranes from normotensive and spontaneously hypertensive rats, after chemical sympathectomy, suggests the presence of presynaptic secretin receptors.

By Pierre Chatelain, Patrick Robberecht, Jean Claude Camus, Philippe De Neef, Magali Waelbroeck, Joseph Roba and Jean Christophe

Abstract

Normotensive (WKY) and spontaneously hypertensive (SHR) male adult rats were sacrificed 2 and 3 weeks after 6-hydroxydopamine treatment. Untreated WKY and SHR rats served as controls. In rat heart membranes from WKY rats, 6-hydroxydopamine treatment increased guanosine 5'-O-(2-3-imido)-triphosphate (Gpp(NH)p)-, NaF-, D,L-isoproterenol- and glucagon-stimulated adenylate cyclase activities by 18-38% while secretin stimulation was unaffected. In heart membranes from SHR rats, Gpp(NH)p, NaF, D,L-isoproterenol, or glucagon stimulation of the enzyme was similarly increased by 14-38% whilst the low secretin responsiveness which is characteristic of these animals decreased even further (by 24-47%). These results are consistent with: (1) an up regulation of postsynaptic beta-adrenergic receptors coupled to adenylate cyclase after degeneration of adrenergic nerves, and (2) a differential response of secretin receptors coupled to adenylate cyclase in the two strains of rats: there was no change in WKY rats and a decreased response in SHR rats. The possible presence and contribution of presynaptic secretin cardiac receptors is considered.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe

Topics: Sciences bio-m├ędicales et agricoles, Adenylate Cyclase -- metabolism, Animals, Blood Pressure -- drug effects, Body Weight -- drug effects, Heart -- drug effects, Heart Rate -- drug effects, Hydroxydopamines -- pharmacology, Hypertension -- enzymology, Male, Membranes -- enzymology, Myocardium -- enzymology, Organ Size -- drug effects, Oxidopamine, Rats, Rats, Inbred Strains, Receptors, Cell Surface -- metabolism, Receptors, G-Protein-Coupled, Receptors, Gastrointestinal Hormone, Sympathectomy, Chemical, Time Factors
Publisher: 'Elsevier BV'
Year: 1983
DOI identifier: 10.1016/0014-2999(83)90147-4
OAI identifier: oai:dipot.ulb.ac.be:2013/148243
Provided by: DI-fusion
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