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Enzymatic C-terminal amidation of amino acids and peptides

By T. Nuijens, E. Piva, J.A.W. Kruijtzer, D.T.S. Rijkers, R.M.J. Liskamp and P.J.L.M. Quaedflieg


Herein, we describe two versatile and high yielding enzymatic approaches for the conversion of semi-protected amino acid and peptidyl C-terminal α-carboxylic acids into their corresponding amides. In the first approach, the lipase Candida antarctica lipase-B (Cal-B), and in the second approach, the protease Subtilisin A, are used, respectively. We found that by using the ammonium salt of the α-carboxylic acid instead of separate ammonia sources, the enzymatic amidation reactions proceeded much faster without side reactions and gave near to quantitative yields of products

Publisher: 'Elsevier BV'
Year: 2012
DOI identifier: 10.1016/j.tetlet.2012.05.039
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Provided by: Enlighten
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