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Holocentric Chromosomes of Luzula elegans Are Characterized by a Longitudinal Centromere Groove, Chromosome Bending, and a Terminal Nucleolus Organizer Region

By S. Heckmann, E. Schroeder-Reiter, K. Kumke, L. Ma, Kiyotaka Nagaki, M. Murata, Gerhard Wanner and A. Houben


The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability. Copyright (C) 2011 S. Karger AG, Basel

Topics: Medizin, ddc:610
Publisher: Ludwig-Maximilians-Universität München
Year: 2011
DOI identifier: 10.1159/000327713
OAI identifier:
Provided by: Open Access LMU

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  1. (2009). AF: Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N.
  2. (2007). al: CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley.
  3. al: Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain.
  4. (2010). al: Neocentrics and holokinetics (holocentrics): chromosomes out of the centromeric rules.
  5. (1979). Bedbrook JR: Cloning and characterization of ribosomal RNA genes from wheat and barley.
  6. (2001). BJ: HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes.
  7. (2008). Chromosomal phylogeny and karyotype evolution in x = 7 crucifer species (Brassicaceae).
  8. (2000). Chromosome analysis and molecular characterization of highly repeated DNAs in the aphid Acyrthosiphon pisum (Aphididae, Hemiptera). Genetica 108: 197–
  9. (2004). Cleveland DW: Structural determinants for generating centromeric chromatin.
  10. DW: An epigenetic mark generated by the incorporation of CENP-A into centromeric nucleosomes.
  11. (2003). et al: KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans .
  12. (1995). F u c h s J , B ran d es A , Sc h u b e rt I : T e l o m e r e s e -quence localization and karyotype evolution in higher plants. Plant Syst Evol 196:
  13. (2008). GH: Epigenetic regulation of centromeric chromatin: old dogs, new tricks?
  14. (1999). Henikoff S: A histone-H3-like protein in C. elegans .
  15. (2001). Here, there, and everywhere: kinetochore function on holocentric chromosomes.
  16. (2004). Heterochromatin and rDNA sites distribution in the holocentric chromosomes of Cuscuta approximata Bab.
  17. (2006). JA: Sensitive fluorescence in situ hybridization signal detection in maize using directly labeled probes produced by high concentration DNA polymerase nick translation.
  18. (2009). Key players in chromosome segregation in Caenorhabditis elegans .
  19. (1984). Localization of the ribosomal genes in Caenorhabditis elegans chromosomes by in situ hybridization using biotinlabeled probes.
  20. (1994). M a r t i n R , B u s c h W , H e r r m a n n R G , W a n n e r G: Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy.
  21. (1999). Manicardi GC: Cytogenetic analysis of the holocentric chromosomes of the aphid Schizaphis graminum . Hereditas 131: 185–190
  22. (2005). Marec F: Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes.
  23. (1999). MB: HCP-1, a protein involved in chromosome segregation, is localized to the centromere of mitotic chromosomes in Caenorhabditis elegans .
  24. (1993). MD: Comparison of plant telomere locations using a PCR-generated synthetic probe.
  25. (1972). Okada TA: Holocentric chromosomes in Oncopeltus : kinetochore plates are present in mitosis but absent in meiosis.
  26. (2004). Papeschi AG: Nucleolus organizing regions and semi-persistent nucleolus during meiosis in Spartocera fusca (Thunberg) (Coreidae, Heteroptera). Hereditas 140: 105– 111
  27. (1985). Rothfield N: Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma.
  28. (1998). Spindle microtubules and chromosome behaviour in mitosis of Luzula luzuloides , a species with holokinetic chromos o m e s . A c t a B i o l o g i c a C r a c o vi e n s i a S e r i e s
  29. (1971). The ultrastructure of the non-localized kinetochores of Luzula and Cyperus .
  30. (1982). Thomson JN: The kinetochores of Caenorhabditis elegans .
  31. (2005). Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea .

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