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Using bioluminescent imaging to investigate synergism between Streptococcus pneumoniae and influenza A virus in infant mice

By K.R. Short, D.A. Diavatopoulos, P.C. Reading, L.E. Brown, K.L. Rogers, R.A. Strugnell and O.L. Wijburg

Abstract

Item does not contain fulltextDuring the 1918 influenza virus pandemic, which killed approximately 50 million people worldwide, the majority of fatalities were not the result of infection with influenza virus alone. Instead, most individuals are thought to have succumbed to a secondary bacterial infection, predominately caused by the bacterium Streptococcus pneumoniae (the pneumococcus). The synergistic relationship between infections caused by influenza virus and the pneumococcus has subsequently been observed during the 1957 Asian influenza virus pandemic, as well as during seasonal outbreaks of the virus (reviewed in (1, 2)). Here, we describe a protocol used to investigate the mechanism(s) that may be involved in increased morbidity as a result of concurrent influenza A virus and S. pneumoniae infection. We have developed an infant murine model to reliably and reproducibly demonstrate the effects of influenza virus infection of mice colonised with S. pneumoniae. Using this protocol, we have provided the first insight into the kinetics of pneumococcal transmission between co-housed, neonatal mice using in vivo imaging

Topics: IGMD 3: Genomic disorders and inherited multi-system disorders, IGMD 3: Genomic disorders and inherited multi-system disorders N4i 4: Auto-immunity, transplantation and immunotherapy, N4i 1: Pathogenesis and modulation of inflammation, N4i 1: Pathogenesis and modulation of inflammation NCMLS 1: Infection and autoimmunity
Year: 2011
OAI identifier: oai:repository.ubn.ru.nl:2066/96505
Provided by: Radboud Repository
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