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High-performance liquid chromatography determination of mycophenolic acid and its glucuronide metabolite in human plasma

By Christopher E. Jones, Paul J. Taylor and Anthony G. Johnson


Two HPLC-UV assays are reported here: one is a rapid assay for mycophenolic acid (MPA) and the other is a simultaneous assay for MPA and its metabolite mycophenolic acid glucuronide (MPAG). For both methods, plasma samples (500 μl) with added internal standard were acidified and extracted using C solid-phase extraction cartridges. Chromatographic separation was achieved on a C Novapak column using a mobile phase consisting of methanol-0.05% orthophosphoric acid (40:60, v/v) for the rapid MPA assay and 30:70 for the simultaneous MPA and MPAG assay. The assays were linear over the ranges 0.1 to 50.0 mg/l for MPA and 2.8 to 225.8 mg/l for MPAG. Mean absolute recovery for all analytes was >99%. These methods are suitable for therapeutic drug monitoring and pharmacokinetic studies

Topics: Mycophenolic acid, 1600 Chemistry
Publisher: 'Elsevier BV'
Year: 1998
DOI identifier: 10.1016/S0378-4347(97)00678-6
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