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Ionic liquid-assisted separation and determination of selenium species in food and beverage samples by liquid chromatography coupled to hydride generation atomic fluorescence spectrometry

By Alexander Castro Grijalba, Emiliano Franco Fiorentini Chirino and Rodolfo German Wuilloud

Abstract

Different ionic liquids (ILs) were assayed as mobile phase modifiers for the separation and determination of selenite [Se(IV)], selenate [Se(VI)], selenomethionine (SeMet) and Se-methylselenocysteine (SeMeSeCys) by reversed-phase high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry (RP-HPLC-HG-AFS). The use of several ILs: 1-butyl-3-methylimidazolium chloride, 1-hexyl-3-methylimidazolium chloride ([C6mim]Cl), 1-octyl-3-methylimidazolium chloride, 1-dodecyl-3-methylimidazolium bromide, 1-hexadecyl-3-methylimidazolium bromide and tributyl(methyl)phosphonium methylsulphate was evaluated. Also, the effect of pH, buffer type and IL concentration on the separation of Se species was studied. Complete separation was attained within 12 min using a C8 column and a gradient performed with a mobile phase containing 0.1% (v/v) [C6mim]Cl at pH 6.0. The proposed method allows the separation of inorganic and organic Se species in a single chromatographic run, adding further benefits over already reported methods based on RP-HPLC. In addition, the influence of ILs on the AFS signals of each Se species was evaluated and a multivariate methodology was used for optimization of AFS sensitivity. The limits of detection were 0.92, 0.86, 1.41 and 1.19 μg L−1 for Se(IV), Se(VI), SeMet and SeMeSeCys, respectively. The method was successfully applied for speciation analysis of Se in complex samples, such as wine, beer, yeast and garlic.Fil: Castro Grijalba, Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fiorentini Chirino, Emiliano Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Wuilloud, Rodolfo German. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentin

Topics: ATOMIC FLUORESCENCE SPECTROSCOPY, BEVERAGE, FOOD, IONIC LIQUID, REVERSED-PHASE CHROMATOGRAPHY, SELENIUM SPECIES, Otras Ciencias Químicas, Ciencias Químicas, CIENCIAS NATURALES Y EXACTAS
Publisher: 'Elsevier BV'
Year: 2017
DOI identifier: 10.1016/j.chroma.2017.02.045
OAI identifier: oai:ri.conicet.gov.ar:11336/59308
Provided by: CONICET Digital
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