Dendritic Mesoporous Silica Nanoparticles with Abundant Ti<sup>4+</sup> for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity


Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti<sup>4+</sup> (denoted DMSNs@PDA-Ti<sup>4+</sup>) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti<sup>4+</sup> with a high surface area (362 m<sup>2</sup> g<sup>–1</sup>), a large pore volume (1.37 cm<sup>3</sup> g<sup>–1</sup>), and a high amount of chelated Ti<sup>4+</sup> (75 μg mg<sup>–1</sup>). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO<sub>2</sub>@PDA-Ti<sup>4+</sup>) and commercial TiO<sub>2</sub>, DMSNs@PDA-Ti<sup>4+</sup> showed better selectivity and a lower detection limit (0.2 fmol/μL). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95%) enabled by DMSNs@PDA-Ti<sup>4+</sup>, better than those in previous reports

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oaioai:figshare.com:article/6450188Last time updated on 8/13/2018

This paper was published in FigShare.

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