Determination of kinetic constants for variants A7P and I58V using isoAsp-DSIP as methyl acceptor.


<p>All assays were carried out in triplicate as described in Materials and methods. For WT (both panels) and A7P (top panel), isoAsp-DSIP was varied over the range of 3–30 μM. For I58V (bottom panel), isoAsp-DSIP was varied over the range of 5–100 μM, but data points for 100 μM are not shown. Initial rates measured at each concentration of isoAsp-DSIP are superimposed on a curve defined by the Michaelis-Menten equation using values of Vmax and Km calculated via the Direct Linear Plot method of Eisenthal and Cornish-Bowden [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198266#pone.0198266.ref017" target="_blank">17</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198266#pone.0198266.ref018" target="_blank">18</a>]. The colored vertical dashed lines intersect the X-axis at the corresponding value of the calculated Km for isoAsp-DSIP.</p

Similar works

Full text

oaioai:figshare.com:article/6403736Last time updated on 8/13/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.