oaioai:figshare.com:article/6403736

Determination of kinetic constants for variants A7P and I58V using isoAsp-DSIP as methyl acceptor.

Abstract

<p>All assays were carried out in triplicate as described in Materials and methods. For WT (both panels) and A7P (top panel), isoAsp-DSIP was varied over the range of 3–30 μM. For I58V (bottom panel), isoAsp-DSIP was varied over the range of 5–100 μM, but data points for 100 μM are not shown. Initial rates measured at each concentration of isoAsp-DSIP are superimposed on a curve defined by the Michaelis-Menten equation using values of Vmax and Km calculated via the Direct Linear Plot method of Eisenthal and Cornish-Bowden [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198266#pone.0198266.ref017" target="_blank">17</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198266#pone.0198266.ref018" target="_blank">18</a>]. The colored vertical dashed lines intersect the X-axis at the corresponding value of the calculated Km for isoAsp-DSIP.</p

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oaioai:figshare.com:article/6403736Last time updated on 8/13/2018

This paper was published in FigShare.

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