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Ng_1063 inhibits human lysozyme killing activities <i>in vitro</i>.

By Stephanie A. Ragland (4453225), Marίa V. Humbert (5485322), Myron Christodoulides (142926) and Alison K. Criss (4453222)

Abstract

<p>A. <i>M</i>. <i>luteus</i> was exposed to vehicle control, 2 μg/mL human lysozyme alone, or 2 μg/mL human lysozyme with increasing concentrations of recombinant 1063 protein (r1063 concentrations indicated in parentheses). Loss of absorbance at λ595 nm, indicating bacterial lysis, was measured over time. <i>n</i> = 3 biological replicates. Differences between vehicle control and lysozyme with 2 μg/mL r1063 were not significant at any time point. B. MS11 WT and <i>ΔltgAΔltgD</i> Gc were exposed to 1.5 μg/mL human lysozyme (HL), which had been left untreated or pretreated with r1063 (0.75 μg/mL final in assay) or recombinant MIP (rMIP, 0.75 μg/mL final in assay), for 5hr. Percent Gc survival was determined by dividing the CFU/mL at 5 hr by the CFU/mL at 0 hr and normalized to the vehicle control (100%). <i>n</i> = 6–15 biological replicates. C. WT, <i>ΔltgAΔltgD</i>, and <i>ΔltgAΔltgD</i>::<i>1063+</i> complement Gc were exposed to 10 μg/mL human lysozyme (HL) for 3 hr. Gc survival was determined as in Fig. 2B. NS, not significant. <i>n</i> = 3–6 biological replicates. All values are represented as the mean ± SEM. *<i>p</i> < 0.05; two tailed <i>t</i>-test.</p

Topics: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Pharmacology, Ecology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, cell wall-degrading enzyme lysozyme, Gc, protein inhibitors, pathogen Neisseria gonorrhoeae, Ng, lysozyme-sensitive bacterium Micrococcus luteus, infects mucosal sites
Year: 2018
DOI identifier: 10.1371/journal.ppat.1007080.g002
OAI identifier: oai:figshare.com:article/6752456
Provided by: FigShare
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