Summary of our findings and proposed models for oxidative damage and aggregation in VVD.


<p>(A) Absorption of BL by dark-VVD results in an excited triplet state (TS). In the conventional photocycle (gray arrows) the TS decays to the flavin-cysteine adduct state corresponding to lit-VVD, from where spontaneous adduct decay follows. Alternatively (black arrows), the TS decays to the ground state by energy transfer to O<sub>2</sub> with the production of <sup>1</sup>O<sub>2</sub>, which in turn promotes internal chemical damage. This mechanism is expected to occur in other LOV domains. In addition, we discovered that the presence of <i>A</i>. <i>niger</i> catalase (CAT) accelerates photoadduct decay. (B) The aggregation pathway of self-oxidized VVD is shown. The lit-VVD state decays into dark-VVD, followed by VVD dimerization and formation of aggregates (VVD-A) that lose the flavin cofactor. The aggregation process is under kinetic control (governed by the dynamics of the FAD-Cys adduct) and limited by VVD dimer formation. Glycerol, BSA, DTT, GSH and CAT directly interact with VVD, impeding VVD dimerization and subsequent aggregation.</p

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oaioai:figshare.com:article/6846629Last time updated on 8/13/2018

This paper was published in FigShare.

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