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Silaffin Peptides as a Novel Signal Enhancer for Gravimetric Biosensors

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Abstract

Application of biomimetic silica formation to gravimetric biosensors has been conducted for the first time. As a model system, silaffin peptides fused with green fluorescent protein (GFP) were immobilized on a gold quartz crystal resonator for quartz crystal microbalances using a self-assembled monolayer. When a solution of silicic acid was supplied, silica particles were successfully deposited on the Au surface, resulting in a significant change in resonance frequency (i.e., signal enhancement) with the silaffin?GFP. However, frequency was not altered when bare GFP was used as a control. The novel peptide enhancer is advantageous because it can be readily and quantitatively conjugated with sensing proteins using recombinant DNA technology. As a proof of concept, this study shows that the silaffin domains can be employed as a novel and efficient biomolecular signal enhancer for gravimetric biosensors.This research was supported by a grant from Marine Biotechnology Program Funded by Ministry of Land, Transport and Maritime Affairs of Korean Government, Kwangwoon University 2013, and also supported by the Agriculture Research Center (ARC, 710003-03-3-SB120) program of the Ministry for Food, Agriculture, Forestry and Fisheries, Korea. In addition, we wish to express our gratitude to Prof. Sangmin Jeon at Pohang University of Science and Technology (POSTECH) for his valuable advice and discussion

Topics: Silaffin peptides, Biomimetic silica formation, R5 peptide, Quartz crystal microbalances, Signal enhancer
Publisher: Springer
Year: 2013
DOI identifier: 10.1007/s12010-013-0161-y
OAI identifier: oai:repository.hanyang.ac.kr:20.500.11754/44703
Provided by: HANYANG Repository
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