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Biotransformation of uridine monophosphate(UMP) and glucose to uridine diphosphate-glucose(UDPG) by Candida saitoana KCTC7249 cells

By Jung Hun Koh, H S Shin, Y S Kim, Dae Sil Lee and C H Kim

Abstract

The present study investigates the biotransformation of glucose with undine monophosphate (UMP) to obtain sugar nucleotide, UDP-glucose (UDPG), by the dried cells of Candida saitoana KCTC7249. The biotransformation was optimized by varying the concentrations of substrates and phosphate ion. UDPG (24 mM) was biotransformed from 200 mM glucose and 37.5 mM UMP by dried cells of C. saitoana. The glucose yields about 64% UDP-glucose, based on UMP concentration. The addition of glucose-1-phosphate to the reaction mixture accelerated the formation of UDPG from a concentration of UMP. The structure of UDP-glucose obtained was determined with 13C NMR and FAB mass spectra. These results indicate that the yeast-dried cells could be used for the production of nucleotide sugars for donor molecules of complex carbohydrate synthesis.ope

Topics: Uridine diphosphate-glucose, biotransformation, C. saitoana
Publisher: Humana Press
Year: 1996
OAI identifier: oai:http://repository.kribb.re.kr:201005/4655
Provided by: KRIBB Repository
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