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Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance

By Yao-Ting Huang, Jia-Min Chen, Bing-Ching Ho, Zong-Yen Wu, Zong-Yen Wu, Rita C. Kuo and Po-Yu Liu and Po-Yu Liu and Po-Yu Liu

Abstract

Stenotrophomonas acidaminiphila is an aerobic, glucose non-fermentative, Gram-negative bacterium that been isolated from various environmental sources, particularly aquatic ecosystems. Although resistance to multiple antimicrobial agents has been reported in S. acidaminiphila, the mechanisms are largely unknown. Here, for the first time, we report the complete genome and antimicrobial resistome analysis of a clinical isolate S. acidaminiphila SUNEO which is resistant to sulfamethoxazole. Comparative analysis among closely related strains identified common and strain-specific genes. In particular, comparison with a sulfamethoxazole-sensitive strain identified a mutation within the sulfonamide-binding site of folP in SUNEO, which may reduce the binding affinity of sulfamethoxazole. Selection pressure analysis indicated folP in SUNEO is under purifying selection, which may be owing to long-term administration of sulfonamide against Stenotrophomonas

Topics: genome sequencing, Stenotrophomonas acidaminiphila, sulfamethoxazole resistance, Stenotrophomonas, comparative genomics, dihydropteroate synthase, Microbiology, QR1-502
Publisher: Frontiers Media S.A.
Year: 2018
DOI identifier: 10.3389/fmicb.2018.01013/full
OAI identifier: oai:doaj.org/article:e0ee9c9913194b7cb12cf07427a68836
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