Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite
Analysis Revealing Atypical N-Glycosylation Sites
on Albumin and α‑1B-Glycoprotein

Shisheng Sun (125326); Yingwei Hu (1837675); Li Jia (76051); Shadi Toghi Eshghi (1326498); Yang Liu (4212112); Punit Shah (1326501); Hui Zhang (7197)

doi:10.1021/acs.analchem.8b01051.s001

Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α‑1B-Glycoprotein

By Shisheng Sun (125326), Yingwei Hu (1837675), Li Jia (76051), Shadi Toghi Eshghi (1326498), Yang Liu (4212112), Punit Shah (1326501) and Hui Zhang (7197)

Abstract

Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins. In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software. Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated. Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans. In addition, two novel atypical glycosites (with N–X–V motif) were identified and validated from albumin and α-1B-glycoprotein. The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses

Topics: Biochemistry, Medicine, Cell Biology, Genetics, Biotechnology, Immunology, Cancer, Infectious Diseases, Plant Biology, Chemical Sciences not elsewhere classified, site-specific glycosylation analysis, LC-MS, triantennary sialylated N, glycosite, Glycosite Analysis Revealing Atypical N, N-linked, NGAG, glycan, glycoproteomic, serum proteins

Year: 2018

DOI identifier: 10.1021/acs.analchem.8b01051.s001

OAI identifier: oai:figshare.com:article/6205829

Provided by: FigShare

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Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical <i>N</i>-Glycosylation Sites on Albumin and α‑1B-Glycoprotein

Abstract

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