Article thumbnail

Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical <i>N</i>-Glycosylation Sites on Albumin and α‑1B-Glycoprotein

By Shisheng Sun (125326), Yingwei Hu (1837675), Li Jia (76051), Shadi Toghi Eshghi (1326498), Yang Liu (4212112), Punit Shah (1326501) and Hui Zhang (7197)

Abstract

Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins. In this study, we profiled serum <i>N</i>-glycoproteome by our recently developed <i>N</i>-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software. Our data indicated that half of identified <i>N</i>-glycosites were modified by at least two glycans, with a majority of them being sialylated. Specifically, 3/4 of glycosites were modified by biantennary <i>N</i>-glycans and 1/3 of glycosites were modified by triantennary sialylated <i>N</i>-glycans. In addition, two novel atypical glycosites (with N–X–V motif) were identified and validated from albumin and α-1B-glycoprotein. The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses

Topics: Biochemistry, Medicine, Cell Biology, Genetics, Biotechnology, Immunology, Cancer, Infectious Diseases, Plant Biology, Chemical Sciences not elsewhere classified, site-specific glycosylation analysis, LC-MS, triantennary sialylated N, glycosite, Glycosite Analysis Revealing Atypical N, N-linked, NGAG, glycan, glycoproteomic, serum proteins
Year: 2018
DOI identifier: 10.1021/acs.analchem.8b01051.s001
OAI identifier: oai:figshare.com:article/6205829
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.