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The Pib2 complex binds glutamine.

By Hirofumi Ukai (5146058), Yasuhiro Araki (25549), Shintaro Kira (480237), Yu Oikawa (823338), Alexander I. May (5146055) and Takeshi Noda (21623)

Abstract

<p>Recombinant 6His-Pib2 or 6His-MBP protein on Ni-NTA agarose was incubated without or with cell lysates of Δ<i>pib2</i> cells (YKOL4391) for 60 min at 4°C. After washing, the [<sup>3</sup>H]l-glutamine-binding assay was performed as described in Materials and Methods. Unlabeled glutamine was added where indicated. Statistical data are shown as Mean ± SE of three independent experiments. ****p < 0.0001, Student’s <i>t</i>-test. The purified proteins were also separated by SDS-PAGE and visualized by Coomassie staining (right panel).</p

Topics: Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Evolutionary Biology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Biological Sciences not elsewhere classified, TORC 1 activation, Gtr, vacuolar membrane TORC 1, Pib 2 pathway, TORC 1, Pib 2, 2-TORC
Year: 2018
DOI identifier: 10.1371/journal.pgen.1007334.g008
OAI identifier: oai:figshare.com:article/6189824
Provided by: FigShare
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