Retromer-associated actin regulators colocalize with CCV actin patches.
<p><b>(A and B)</b> CCV actin patches colocalize with Arp3, cortactin, WASH, and FAM21. The retromer protein VPS35 also colocalizes with patches whereas N-WASP does not. Vero cells at 3 dpi were immunostained for the indicated proteins. Histogram depicts the means ± SD of ≥ 60 cells for at least 3 independent experiments. Statistical significance was determined using Student’s t-test (****<i>P</i> <0.0001). Colocalization was determined using Pearson’s correlation coefficient. NMII, <i>C</i>. <i>burnetii</i> Nine Mile phase II strain. Scale bar, 5 μm.</p
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Cancer, Infectious Diseases, CCV formation, actin patches, CCV actin patches, actin filaments, Arp, cationic-independent mannose -6-phosphate receptor, glucose transporter 1, GLUT, SNARE, Filamentous actin patches, CI-M 6PR, CCV membrane, HOPS, host retromer function, VPS 35, knockout mouse embryo fibroblasts, VPS 29, CK, VAMP, FAM, burnetii type 4 B secretion, pathogen growth Coxiella burnetii
DOI identifier: 10.1371/journal.ppat.1007005.g004
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