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CCV actin patches facilitate vesicle fusion via docking and clustering of late endocytic vesicles to the CCV membrane.

By Heather E. Miller (3826666), Charles L. Larson (140385) and Robert A. Heinzen (196263)

Abstract

<p><b>(A)</b> LAMP1<sup>+</sup> vesicles fuse with the CCV at actin patches. Live cell images of a Vero cell at 3 dpi expressing lysosome-GFP (LAMP1) and actin-RFP. <b>(B)</b> Latrunculin A (LatA) treatment redistributes LAMP1<sup>+</sup> clusters at actin patches around the CCV to the juxta-nuclear region (white arrows). Live cell images of a Vero cell at 3 dpi expressing lysosome-GFP (LAMP1) and actin-RFP. <b>(C and D)</b> LatA treatment redistributes VAMP7<sup>+</sup> clusters at actin patches to the juxta-nuclear region (white arrows). Vero cells at 3 dpi were treated with LatA for 10 min prior to fixation and immunostaining for F-actin, VAMP7, and NMII. Histogram depicts the mean intensity ± SD of ≥ 60 cells for at least 3 independent experiments. Statistical significance was determined using Student’s t-test (****<i>P</i> <0.0001). NMII, <i>C</i>. <i>burnetii</i> Nine Mile phase II strain. Scale bar, 2.5 μm.</p

Topics: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Cancer, Infectious Diseases, CCV formation, actin patches, CCV actin patches, actin filaments, Arp, cationic-independent mannose -6-phosphate receptor, glucose transporter 1, GLUT, SNARE, Filamentous actin patches, CI-M 6PR, CCV membrane, HOPS, host retromer function, VPS 35, knockout mouse embryo fibroblasts, VPS 29, CK, VAMP, FAM, burnetii type 4 B secretion, pathogen growth Coxiella burnetii
Year: 2018
DOI identifier: 10.1371/journal.ppat.1007005.g002
OAI identifier: oai:figshare.com:article/6157238
Provided by: FigShare
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