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Characterization and determination of chlorophacinone in plasma by ion chromatography coupled with ion trap electrospray ionization mass spectrometry

By Xiao-kun OuYanga, Xiao-hong Chen, Yong-qing Yan and Mi-cong Jin

Abstract

Plasmatic chlorophacinone is commonly measured with liquid chromatographic assay, which convenient but lacks sensitivity and selectivity and usually requires ion pair reagents to reduce the chromatographic tailed peak. In this paper, a novel method using eluent generator reagent-free ion chromatography coupled with electrospray ionization ion trap mass spectrometric detection for the determination of chlorophacinone in plasma has been developed. After samples were extracted with 10% (v/v) methanol in acetonitrile and cleaned by solid-phase extraction, chromatographic separation was performed on an lonPac (R) AS11 analytical column (250 x 4.0 mm) using 40.0 mmol/L KOH containing 10% (v/v) methanol as organic modifier. Quantification was performed by negative electrospray ionization in multiple reaction monitoring mode. The transition m/z 373 -> 201 was for the quantification ion; the transitions m/z 373 -> 172 and m/z 373 -> 145, as well as the isotope ions m/z 375 and m/z 203, were for the qualitative ions. All the method parameters were validated. It was confirmed that this method can be used in clinical diagnosis and forensic toxicology. Copyright (C) 2008 John Wiley & Sons, Ltd.Biochemical Research MethodsBiochemistry & Molecular BiologyChemistry, AnalyticalPharmacology & PharmacySCI(E)0ARTICLE5524-5302

Topics: ion chromatography-mass spectrometry, chlorophacinone, rodenticide, plasma, gradient eluent generator, PERFORMANCE LIQUID-CHROMATOGRAPHY, 8 ANTICOAGULANT RODENTICIDES, SOLID-PHASE EXTRACTION, LC-APCI-MS, ANIMAL-TISSUES, WHOLE-BLOOD, MULTIRESIDUE ANALYSIS, DIPHACINONE, RESIDUES, SERUM
Publisher: 生物医学色谱法
Year: 2009
DOI identifier: 10.1002/bmc.1148
OAI identifier: oai:localhost:20.500.11897/245867
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