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Differential chemotactic potential of mouse platelet basic protein for thymocyte subsets

By WX Fu, SY Gong, XP Qian, Y Li, ML Zhu, XY Dong, Y Li and WF Chen

Abstract

Mouse platelet basic protein (CXCL7/mPBP) was cloned from thymic stromal cells and further identification indicated that it was expressed in thymic monocytes/macrophages (Mo/Mphis). Recombinant mPBP was chemoattractive for target cells of polymorphonuclear leucocytes, peritoneal Mo/Mphis and splenic lymphocytes with distinct potencies. CXCR2 was identified to be a cognate receptor for mPBP. Mouse thymocyte subsets of CD4(-)CD8(-) double-negative (DN), CD4(+)CD8(+) double-positive (DP), CD4(+)CD8(-) single-positive (CD4SP) and CD4(-)CD8(+) single-positive (CD8SP) expressed cell surface CXCR2 with different positive percentages and expression levels. mPBP was chemoattractive for thymocyte subsets with the potency order DN>DP> CD8SP>CD4SP, consistent with the levels of CXCR2 expressed on the respective cells. Thus, mPBP in thymus is functionally redundant with chemokine CXCL12/ SDF-1. Moreover, our finding that thymic Mo/Mphis can produce mPBP implies that they may have other functions apart from acting as scavengers in thymus.Biochemistry & Molecular BiologyCell BiologySCI(E)PubMed4ARTICLE151935-19456

Topics: chemokine, chemotaxis, monocyte/macrophage, stromal cell, thymus, MACROPHAGE-DERIVED CHEMOKINE, MEDULLARY EPITHELIAL-CELLS, IL-8 RECEPTOR HOMOLOG, BONE-MARROW, T-CELLS, PROGENITOR CELLS, LINE MTEC1, MURINE, NEUTROPHIL, EXPRESSION
Publisher: cellular and molecular life sciences
Year: 2004
DOI identifier: 10.1007/s00018-004-4137-5
OAI identifier: oai:localhost:20.500.11897/200284
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