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Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated <i>E</i>. <i>coli</i> O157:H7 on <i>Hermetia illucens</i> larvae

By Mahboobeh Kashiri (4994027), Cuauhtemoc Marin (4994024), Raquel Garzón (4994021), Cristina M. Rosell (534141), Dolores Rodrigo (688352) and Antonio Martínez (2450845)

Abstract

<div><p>A chemical and microbiological characterization on <i>Hermetia illucens</i> larvae was carried out as well as an inactivation study of natural contaminating microorganisms and inoculated <i>E</i>. <i>coli</i> O157:H7 in black soldier larvae by using High Hydrostatic Pressure (250 to 400 MPa, for 1.5 to 15 min). <i>Hermetia illucens</i> was mainly composed of proteins (46.49%, d.m.) followed by fat (37.88%, d.m.). Larvae had a high contamination load of Total Aerobic Mesophilic bacteria (AMB) (1.58x10<sup>7</sup> cfu/g) and Enterobacteriaceae (1.15x10<sup>6</sup>cfu/g). The presence of pathogenic microorganism varied: no <i>Listeria</i> spp. were found, but <i>Salmonella</i> (1.15x10<sup>6</sup> cfu/g) and <i>E</i>. <i>coli</i> (7.08x10<sup>5</sup> cfu/g) were detected in the larvae extract. High Hydrostatic Pressure (HHP) was effective against natural contaminating yeasts and molds producing more than 5 log cycle reductions at 400 MPa for any of the times considered (2.5 to 7 min), but a low reduction of total microbial load was achieved. The inactivation level of larvae inoculated with <i>E</i>. <i>coli</i> O157:H7 varied. At 400 MPa for 7 min more than 5 log cycle reductions were achieved. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells, according to the Akaike index, was the Biphasic model.</p></div

Topics: Biochemistry, Microbiology, Ecology, Immunology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, 5 log cycle reductions, inoculated E, coli, High Hydrostatic Pressure, cfu, Hermetia illucens larvae, AMB, 400 MPa, load, microorganism, d.m, inactivation, Total Aerobic Mesophilic bacteria, HHP, min, model
Year: 2018
DOI identifier: 10.1371/journal.pone.0194477
OAI identifier: oai:figshare.com:article/6018122
Provided by: FigShare
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