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Diagnostic tests for antiribosomal p protein antibodies: a comparative evaluation of immunoblotting and ELISA assays

By Anna Ghirardello, Laura Caponi, Franco Franceschini, Sandra Zampieri, Marzia Quinzanini, Raffaele Bendo, Stefano Bombardieri, Pier Franco Gambari and Andrea Doria

Abstract

We compared the clinical sensitivity and specificity of three different methods for the detection of serum antiribosomal P protein (anti-P) antibodies in systemic lupus erythematosus (SLE). Sera from 60 unselected SLE patients, 100 healthy subjects and 100 patients with other rheumatic inflammatory diseases were screened for anti-P antibodies by immunoblotting (IB) on P proteins from Raji cells and by two ELISA assays, one using the C-terminal 22 aminoacid long synthetic peptide (C-22) of P proteins, the other using a multiple antigen peptide (MAP) carrying four copies of the C-terminal 13 aminoacid long P peptide.Anti-P antibodies were found in 20% lupus sera by IB, 16.7% by MAP ELISA and 11.7% by C-22 ELISA. The specificity for SLE diagnosis of the three tests in healthy subjects and other rheumatic diseases was: 100% by IB, 100% (vs healthy subjects) and 97% (vs rheumatic diseases) by C-22 ELISA, 100% by MAP ELISA. The agreement between methods was good; differences in concordance rates were restricted to weak positivities. We observed a high concordance in the results of IB and ELISA methods for anti-P antibody detection. IB on P proteins extracted from human lymphoid cells is more sensitive than both ELISAs; IB and MAP ELISA perform better than the C-22 ELISA in determining weakly positive sera

Topics: Adolescent, Adult, Aged, Antibodies, Female, Humans, Lupus Erythematosus, Systemic, Male, Middle Aged, Ribosomal Proteins, Enzyme-Linked Immunosorbent Assay, Immunoblotting, Protozoan Proteins
Year: 2002
DOI identifier: 10.1006/jaut.2002.0595
OAI identifier: oai:iris.unibs.it:11379/501989
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