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An agar dilution method for the determination of the minimum inhibitory concentration of essential oils

By Shane G Griffin, Julie L Markham and David N Leach

Abstract

Abstract Methods for the measurement of the minimum inhibitory concentration (MIC) of antibacterial agents have been developed for water-soluble substances and require adaptation for use with water-insoluble essential oils. This paper reports a standardized agar dilution MIC method, using 0.5% v/v Tween 20 as a dispersant, which provides a reliable and reproducible technique. The method was tested using two Melaleuca alternifolia oil samples with two different levels of terpinen-4-ol (37% and 45% v/v). The MIC values of the tea tree oil samples against a wide selection of bacteria, moulds and yeast are reported

Topics: Plant Sciences
Publisher: ePublications@SCU
Year: 2000
DOI identifier: 10.1080/10412905.2000.9699509
OAI identifier: oai:epubs.scu.edu.au:cpp_pubs-1078
Provided by: ePublications@SCU
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