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In (A), a schematic representation of the CCR3 exon region used for electrophoretic mobility shift assays is shown

By Nives Zimmermann (27124), Jessica L Colyer (27125), Laura E Koch (27126) and Marc E Rothenberg (27127)

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Analysis of the CCR3 promoter reveals a regulatory region in exon 1 that binds GATA-1"</p><p>BMC Immunology 2005;6():7-7.</p><p>Published online 4 Apr 2005</p><p>PMCID:PMC1080127.</p><p>Copyright © 2005 Zimmermann et al; licensee BioMed Central Ltd.</p> E1-FL is the exon 1 full-length probe. Overlapping short probes are called E1-A, E1-B and E1-C, respectively. The DNA sequence is shown with GATA sites boxed. In (B), EMSA assay with the full-length probe and extracts from eosinophilic AML14.3D10 cells is shown. As cold competitors (CC), the full-length probe and short probes E1-A through C were used. The arrow depicts the specific band. A representative experiment, of three similar experiments, is shown

Topics: Uncategorised, schematic, representation, exon, region, electrophoretic, mobility, shift, assays, shown
Year: 2011
DOI identifier: 10.6084/m9.figshare.50785.v1
OAI identifier: oai:figshare.com:article/50785
Provided by: FigShare
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