In (A), a schematic representation of the CCR3 exon region used for electrophoretic mobility shift assays is shown

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Analysis of the CCR3 promoter reveals a regulatory region in exon 1 that binds GATA-1"</p><p>BMC Immunology 2005;6():7-7.</p><p>Published online 4 Apr 2005</p><p>PMCID:PMC1080127.</p><p>Copyright © 2005 Zimmermann et al; licensee BioMed Central Ltd.</p> E1-FL is the exon 1 full-length probe. Overlapping short probes are called E1-A, E1-B and E1-C, respectively. The DNA sequence is shown with GATA sites boxed. In (B), EMSA assay with the full-length probe and extracts from eosinophilic AML14.3D10 cells is shown. As cold competitors (CC), the full-length probe and short probes E1-A through C were used. The arrow depicts the specific band. A representative experiment, of three similar experiments, is shown