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RNase protection assay on mitochondrial (−)strand transcripts mapping around the cyt b/ND1-binding site in DmTTF-depleted cells

By Marina Roberti (16676), Francesco Bruni (16677), Paola Loguercio Polosa (16678), Maria Nicola Gadaleta (16679) and Palmiro Cantatore (16680)

Abstract

<p><b>Copyright information:</b></p><p>Taken from "The termination factor DmTTF regulates mitochondrial transcription"</p><p>Nucleic Acids Research 2006;34(7):2109-2116.</p><p>Published online 28 Apr 2006</p><p>PMCID:PMC1450328.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Schematic representation of digestion products using probes Ribo-1 (295 nt) and Ribo-2 (218 nt). Riboprobes (grey bold arrows), mature transcripts (continuous arrows) and read-through transcripts (dotted arrows) are indicated above the cyt b/ND1 region map. Dashed regions indicate non-coding sequences. () Total cellular RNA (50 µg), extracted from untreated (control) and DmTTF-dsRNA treated (RNAi) D.Mel-2 cells, was hybridized with about 1.5 × 10 c.p.m. of Ribo-1 and Ribo-2 probes and digested with RNase A and T1. Digestion products were denatured and run on a 10% polyacrylamide/7 M urea gel. Y, sample containing 50 µg of yeast total RNA. M, Decade RNA marker (Ambion)

Topics: Cell Biology, rnase, dmttfdepleted, site, cyt, mapping, transcripts, mitochondrial, assay, protection, cells
Year: 2011
DOI identifier: 10.6084/m9.figshare.53965.v1
OAI identifier: oai:figshare.com:article/53965
Provided by: FigShare
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