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Generation of FGF reporter transgenic zebrafish and their utility in chemical screens-4

By Gabriela A Molina (75981), Simon C Watkins (19859) and Michael Tsang (41061)

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Generation of FGF reporter transgenic zebrafish and their utility in chemical screens"</p><p>http://www.biomedcentral.com/1471-213X/7/62</p><p>BMC Developmental Biology 2007;7():62-62.</p><p>Published online 6 Jun 2007</p><p>PMCID:PMC1904198.</p><p></p>e bottom right. The protein targets for these compounds are listed in the top right hand corner. Embryo incubated in 0.5% DMSO as control. Increasing doses of SU5402 suppressed d2EGFP expression in the MHB (red bracket), trigeminal ganglia (red arrow) and dorsal retina (red arrowhead). Increasing doses of SU5416, a non-specific inhibitor of VEGFRs, suppressed FGF signalling. Oxindole I another related VEGFR inhibitor also suppressed d2EGFP fluorescence in transgenic embryos. In contrast, two compounds with similar chemical structure SU4312 and SU11652, did not block FGF signalling. Likewise, two unrelated inhibitors of PDGFR and VEGFR, SU1433 and SU1498 also failed to suppress d2EGFP expression. PP2 and SU6656, two Src Kinase inhibitors suppressed FGF signalling in transgenic embryos

Topics: Genetics, generation, fgf, reporter, transgenic, zebrafish, utility, chemical
Year: 2011
DOI identifier: 10.6084/m9.figshare.57821.v1
OAI identifier: oai:figshare.com:article/57821
Provided by: FigShare
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