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The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues-3

By Rakesh Singh Dhanda (85235), Sofia Rondin Lindberg (85236) and Inge Olsson (85237)

Abstract

and ETO mutants lacking NHR1 (N1), NHR2 (N2), NHR3 (N3), NHR4 (N4) or ETO lacking 30 amino-terminal aminoacids (30) as described in Material and Methods. IP was performed with α-SIN3B followed by Western blotting with α-ETO (α-E). The ETO mutant lacking NHR2 was not pulled down by hSIN3B (lane 3). Both IP and Western blotting with α-SIN3B (α-S) confirmed expression of hSIN3B (arrowhead). Western blotting performed with α-E demonstrated expression of all the mutants (arrowhead). IP was performed with α-SIN3B or α-ETO followed by Western blotting. The presence in the left panel of ETO in lane 1 but not in lane 4 (lacking 30 amino-terminal aminoacids) showed that the ETO amino-terminus is required for interaction with hSIN3B. Reciprocal experiment showed that hSIN3B co-precipitated ETO (right panel, lane 2) but not ETO – 30 aminoacids (right panel, lane 5). Arrowheads show the position of ETO, ETO – 30 aas and hSIN3B. Lower left and right panels show input of ETO and hSIN3B in 2% of IP lysate. The positions of the molecular weight markers are indicated at the left.<p><b>Copyright information:</b></p><p>Taken from "The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues"</p><p>http://www.biomedcentral.com/1471-2199/9/8</p><p>BMC Molecular Biology 2008;9():8-8.</p><p>Published online 19 Jan 2008</p><p>PMCID:PMC2266940.</p><p></p

Topics: Uncategorised, human, corepressor, forms, nucleolar, complex, leukemiaassociated, eto
Year: 2011
DOI identifier: 10.6084/m9.figshare.75049.v1
OAI identifier: oai:figshare.com:article/75049
Provided by: FigShare
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