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(A) Surface expression of CXCR2 on GR-1–positive leukocytes of ST3Gal-IV and WT mice was analyzed by flow cytometry

By David Frommhold (85178), Andreas Ludwig (92950), M. Gabriele Bixel (92951), Alexander Zarbock (92952), Inna Babushkina (92953), Melitta Weissinger (92954), Sandra Cauwenberghs (92955), Lesley G. Ellies (42451), Jamey D. Marth (92956), Annette G. Beck-Sickinger (92957), Michael Sixt (92958), Bärbel Lange-Sperandio (92959), Alma Zernecke (92960), Ernst Brandt (92961), Christian Weber (86566), Dietmar Vestweber (92962), Klaus Ley (8593) and Markus Sperandio (85182)


Leukocytes were incubated with an allophycocyanin-labeled GR-1 mAb, a PE-labeled anti-CXCR2 mAb, and a PE-labeled isotype control. (B) For ligand binding studies, leukocytes from the peripheral blood of WT ( = 4), ST3Gal-IV ( = 3), and CXCR2 ( = 3) mice were incubated with different dilutions of CF-CXCL8 and subsequently analyzed for bound fluorescence by flow cytometry. Peripheral blood leukocytes from WT mice were treated with 100 mU/ml neuraminidase for 60 min and investigated for CF-CXCL8 binding. Data are given as mean ± SD. P < 0.05 for WT versus ST3Gal-IV, CXCR2, and WT + Neu for 30 and 90 nM CF-CXCL8, respectively. MFI, mean fluorescence intensity.<p><b>Copyright information:</b></p><p>Taken from "Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1435-1446.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413039.</p><p></p

Topics: Uncategorised, expression, leukocytes, mice, analyzed, flow, cytometry
Year: 2011
DOI identifier: 10.6084/m9.figshare.82550.v1
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Provided by: FigShare
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