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Immunoblot showing levels of wild-type and mutant TSC1-FLAG proteins and endogenous TSC2 in 97-1 cell lines

By Louis S. Pymar (89384), Fiona M. Platt (89385), Jon M. Askham (89386), Ewan E. Morrison (89387) and Margaret A. Knowles (89388)

Abstract

() Measurement of TSC1 RNA levels by real time RT–PCR analysis of wild-type and mutant TSC1-FLAG mRNA transcript levels in 97-1 cell lines. RT−ve is reverse transcriptase-negative control, NTC is no template control. TSC1 expression is standardized to SDHA and normalized to the 97-1 Neo cell line.<p><b>Copyright information:</b></p><p>Taken from "Bladder tumour-derived somatic missense mutations cause loss of function via distinct mechanisms"</p><p></p><p>Human Molecular Genetics 2008;17(13):2006-2017.</p><p>Published online 7 Apr 2008</p><p>PMCID:PMC2427143.</p><p>© The Author 2008</p

Topics: Genetics, immunoblot, levels, wildtype, mutant, proteins, endogenous, cell
Year: 2012
DOI identifier: 10.6084/m9.figshare.76132.v2
OAI identifier: oai:figshare.com:article/76132
Provided by: FigShare
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