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The interaction of CaMKII with spinophilin increases with age.

By Anthony J. Baucum II (184238), Stefan Strack (184241) and Roger J. Colbran (132826)

Abstract

<p><i>A.</i> TSFs isolated from PND21 or adult striatum were immunoblotted for spinophilin, PP1 catalytic subunit (PP1c), CaMKIIβ, CaMKIIα, or PSD-95. <i>B.</i> Spinophilin was immunoprecipitated from TSFs isolated from PND21 (P21) or adult animals. Precipitates were immunoblotted for spinophilin, PP1c, CaMKIIβ, or CaMKIIα. <i>C.</i> Striatal TSFs from PND21 or adult mice were immunoblotted for phospho-Thr286/7 and total CaMKII. Phospho-Thr286/7 immunoreactivity was normalized to total CaMKIIβ or CaMKIIα levels, respectively. All values were normalized to the mean at PND21, and then expressed as the mean±S.E.M from analyses of the indicated number of animals. * P<0.05, **P<0.01, ***P<0.001.</p

Topics: Biochemistry, Cell Biology, Molecular Biology, Neuroscience, Physiology, Evolutionary Biology, Developmental Biology, camkii, spinophilin, increases
Year: 2012
DOI identifier: 10.1371/journal.pone.0031554.g007
OAI identifier: oai:figshare.com:article/354539
Provided by: FigShare
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