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Chimeric kinesin proteins verify that the UncA tail is sufficient for microtubule specificity.

By Constanze Seidel (182180), Nadine Zekert (182186) and Reinhard Fischer (182192)

Abstract

<p>(<b>A</b>) Scheme for the creation of chimera of kinesin 1 (yellow), KinA, and kinesin 3, UncA (green). (<b>B</b>) Localization of GFP-UncA (SNZ2) and (<b>C</b>) mRFP-KinA (SCS6-NZ). (<b>D</b>) Time lapse of the KinA–UncA chimeric protein (SCoS23). Arrows indicate a moving vesicle. Vesicles also accumulate at the tip of hyphae, similar to the UncA localization. (<b>E</b>) Growth comparison of WT, <i>ΔuncA</i> and the <i>ΔuncA</i> strain complemented with the KinA-UncA chimera (SCoS23). The fusion protein can restore the <i>ΔuncA</i> phenotype. (<b>F</b>) Localization pattern of KinA<sup>rigor</sup>-UncA chimera (SCoS24) labeled with GFP in the <i>ΔuncA</i> strain, under the control of the <i>uncA</i> promoter. The chimera shows the same specificity as UncA<sup>rigor</sup>. (<b>G</b>) In contrast UncA<sup>rigor</sup>-KinA chimera (SCoS44) in <i>ΔuncA</i>, labeled with GFP, under the control of the <i>uncA</i> promoter do not label MT subpopulations. Hyphae are 3 µm in diameter.</p

Topics: Biochemistry, Microbiology, Cell Biology, Developmental Biology, kinesin, proteins, verify, unca, microtubule
Year: 2012
DOI identifier: 10.1371/journal.pone.0030976.g003
OAI identifier: oai:figshare.com:article/349796
Provided by: FigShare
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