Asymmetric pectocin M1 spot test inhibition zones and growth enhancement due to plant-like ferredoxin, but not adrenodoxin

Abstract

<p>a) Asymmetric pectocin M1 inhibition zones against <i>P. atrosepticum</i> LMG2386 due to plant-like ferredoxin containing proteins. Protein concentration for spots were as follows: pectocin M1 1.2 mg ml⁻¹, spinach ferredoxin 20 mg ml⁻¹, adrenodoxin 30 mg ml⁻¹, pectocin M2 10 mg ml⁻¹, pectocin M1 D222A 5.6 mg ml⁻¹ b) Growth enhancement of <i>P. carotovorum subspp. carotovorum</i> LMG 2410 by plant-like ferredoxin containing proteins. Protein concentrations for spots were as follows: spinach ferredoxin 20 mg ml⁻¹, adrenodoxin 30 mg ml⁻¹, Spinach ferredoxin 20 mg ml⁻¹ :pectocin M1 1.2 mg ml⁻¹. Pectocin M1 causes asymmetry in zone of enhancement due to spinach ferredoxin in LMG 2410 (400 µM 2,2′-bipyridine) presumably through competition for a common receptor. Pectocin M1 does not enhance growth of this strain under these conditions.</p