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Asymmetric pectocin M1 spot test inhibition zones and growth enhancement due to plant-like ferredoxin, but not adrenodoxin

By Rhys Grinter (178616), Joel Milner (178619) and Daniel Walker (178621)

Abstract

<p>a) Asymmetric pectocin M1 inhibition zones against <i>P. atrosepticum</i> LMG2386 due to plant-like ferredoxin containing proteins. Protein concentration for spots were as follows: pectocin M1 1.2 mg ml<sup>−1</sup>, spinach ferredoxin 20 mg ml<sup>−1</sup>, adrenodoxin 30 mg ml<sup>−1</sup>, pectocin M2 10 mg ml<sup>−1</sup>, pectocin M1 D222A 5.6 mg ml<sup>−1</sup> b) Growth enhancement of <i>P. carotovorum subspp. carotovorum</i> LMG 2410 by plant-like ferredoxin containing proteins. Protein concentrations for spots were as follows: spinach ferredoxin 20 mg ml<sup>−1</sup>, adrenodoxin 30 mg ml<sup>−1</sup>, Spinach ferredoxin 20 mg ml<sup>−1</sup> :pectocin M1 1.2 mg ml<sup>−1</sup>. Pectocin M1 causes asymmetry in zone of enhancement due to spinach ferredoxin in LMG 2410 (400 µM 2,2′-bipyridine) presumably through competition for a common receptor. Pectocin M1 does not enhance growth of this strain under these conditions.</p

Topics: Biochemistry, Microbiology, Biotechnology, Ecology, Developmental Biology, Plant Biology, pectocin, m1, inhibition, zones, enhancement, plant-like
Year: 2012
DOI identifier: 10.1371/journal.pone.0033033.g004
OAI identifier: oai:figshare.com:article/340544
Provided by: FigShare
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